相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保质期:
一年
- 英文名:
GenJet™ In Vitro DNA Tranfection Reagent for SK-OV-3 Cells
- 库存:
现货 大量
- 供应商:
济南美中清水湾生物科技有限公司
- 保存条件:
4°C
GenJet™ DNA In Vitro Tranfection Reagent for SKOV3 cell is pre-optimized for transfecting SKOV3 cells.
Refer to the following optimal transfection conditions for maximal transfection efficiency on SKOV3 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
| Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium After Transfection Maximal Efficiency Transfection Results: Reporter Gene Plasmid Efficiency (GFP %) |
90% DMEM with 4.5 g/L glucose, 10% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes Yes, 18~24 Hours After Transfection 48 hours EGFP pEGFP-N3 (CMV promoter) 82% |
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关专题 总有一种转染方法适合你 近日,罗氏公司新推出了两款高性能的DNA转染试剂,为其细胞分析产品线再添新军。这两款名为X-tremeGENE 9和X-tremeGENE HP的转染试剂沿用了X-tremeGENE siRNA 转染试剂的前缀,应该与大名鼎鼎的Fugene 6不属于一类。据罗氏介绍,这两个产品是多组分的独特试剂,并非脂质体。它们能够提供高的转染效率、重复性和细胞存活率。 这两款新产品都融合了罗氏二十
Synthetic microRNA Targeting Glioma-associated Antigen-1 Protein
inhibited proliferation of Gli-1+ ovarian (SK-OV-3) and pancreatic (MiaPaCa-2) tumor cells by delaying cell division and activating late apoptosis in MiaPaCa-2 cells. Here, we describe the design of effective miRNA sequences and their applications as anti
NEB 发布:GenomONE™ 为诱导 iPSC 形成「保驾护航」
间充质干细胞(MSC, mesenchymal stem cells)是干细胞家族的重要成员,属于多能干细胞,因其具有多向分化潜能、造血支持和促进干细胞植入、免疫调控和自我复制等特点而日益受到人们的关注。诱导性多能性的间充质干细胞(iPMSCs)是药物筛选、再生医学和细胞治疗的新型候选药物。然而,用于生成骨髓间充质干细胞的诱导性多能性间充质干细胞(iPSC),在导入编码基因的转录因子中,往往伴随有在靶细胞基因组的插入时发生突变的风险。 由于近年来对骨髓间充质干细胞认知地深入,在造血系统、免疫
技术资料暂无技术资料 索取技术资料








