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文献和实验【信息】Northstar BioProducts(NSBP)
, (Streptococcus sp.), EC 3.2.1.18 1 unit Acetylneuraminyl hydrolase (Sialidase). Sterilized preparation of 120052-1 by membrane filtration Lyophilized powder. Activity: ≥50 units/mg. M.W.: 85,000 (gel filtration) 120055-1 (2-3) Neuraminidase
Through a combination of analytical HPLC, electrospray LC-MS, and/or MALDI-TOF, determine the purity of the crude product and whether the desired molecular weight is present. 2) Prepare a ˜5-10 mg/mL solution of the dry peptoid powder in water with minimal
, lyophilized Tris/guanidine buffer, pH 8.0 (see recipe ) or 0.1 M Tris·Cl, pH 8.0 50 mM dithiothreitol (DTT; 7.7 mg/ml
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