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白酶识别类泛素SUMO结构域的三级结构，并在SUMO结构域C端的x–Gly–Gly–x序列中，在Gly-Gly键后水解肽键。除了切割天然SUMO修饰蛋白外，SUMO蛋白酶还用于切割重组SUMO融合蛋白。
这种生物素标记的SUMO蛋白酶产品旨在用于柱上切割SUMO融合蛋白。该方法特异性地从柱结合的SUMO融合蛋白中切割目标蛋白，保留SUMO结构域与亲和柱（例如，Ni-NTA柱）结合，仅洗脱目标蛋白。
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文献和实验相关实验
FRET-Based In Vitro Assays for the Analysis of SUMO Protease Activities
, real-time analysis of the three major SUMO protease activities.
In this chapter we describe a novel, sensitive, homogenous high throughput reporter-based in vitro assay for SUMO protease activity developed by Progenra, Inc. A reporter construct was created by fusing His6 -tagged small ubiquitin
in the nucleus in human cell cultures we designed several RNA-biotin based pulldown assays which can be used alone or in combination with other known assays such as ChIP and Flag-tagged pulldown assays. Three protocols are explained
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SUMO Protease, Biotin tagged
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