引物设计求教 急
丁香园论坛
1090
求助 帮设计引物
求助 哪为高手帮我设计一下以下序列引物 要求 长度大于等于20bp 上游引物最好在1－100 下游1449－2046 不需要酶切位点 谢谢ggctccgcagagtgaccctttttcttggaaaagcggtggcgagagaagtgaaggtggctgttgggtagggagtcaagact
cctggaaggtggagagggtggcgggaggatgagctcgccgggcacagagagcgcagggaagagcctgcagtaccgagtgg
accacctgctcagcgccgtggagagcgagctgcaggcgggcagcgagaagggagaccccaccgaacgcgaactgcgagtg
ggcctggaggagagcgagctgtggctgcgcttcaaggagctaactaacgagatgattgtgaccaagaacggcaggaggat
gttcccggtgctgaaggtaaatgtgtcaggcctggaccccaatgccatgtactctttcttgctggacttcgtgacggctg
acaaccaccgctggaaatatgtgaacggggagtgggtacctgggggcaaaccagagcctcaggcgcccagctgcgtctac
atccacccagactcgcccaattttggggcccactggatgaaggcgcctgtgtctttcagcaaagtcaaactcaccaacaa
gctcaatggagggggacagatcatgttaaactccttgcataagtatgaacctcggattcacatcgtgagagttgggggcc
cgcaacgcatgatcaccagccactgctttcccgagacccagttcatagctgtgactgcctaccagaatgaggagattaca
gcccttaaaattaaatacaacccatttgctaaagccttccttgatgccaaagaaagaaacgaccacaaagatgtaatgga
ggaaccgggggactgccagcagccggggtattcccaatgggggtggcttgttcctggtgctggcaccctctgcccgcctg
ccagctcccaccctcagtttggaggctcgctctctctcccctccacacacggctgtgagaggtacccagctctaaggaac
caccggtcatcgccctaccccagcccctatgctcatcggaacagctctccaacctatgcggacaattcatctgcttgtct
gtccatgctgcagtcccatgataactggtctagcctcggagtgcctggccacaccagcatgctgcctgtgagtcataacg
ccagcccacctactggctctagccagtatcccagtctctggtctgtgagcaatggtaccatcaccccaggctcccagaca
gctggggtgtccaacgggctgggagctcagttctttcgaggctcccctgcacattacacaccactgacgcacacggtctc
agctgccacgtcctcgtcttctggttctccgatgtatgaaggggctgctacagtcacagacatttctgacagccagtatg
acacggcccaaagcctcctcatagcctcgtggacacctgtgtcacccccatctatgtgaattgaactttcctccatgtgc
tgagacttgtaacaaccggtgtcaactggatcttctaggctcaaagtggcaggctcttgggacaagggaaaaataaataa
ataaaagctagatactaacaactccattttcaaataagagcaataatacatgtcctataatcatgttctacagcctcttg
tttgatacctacagtagtgatatgtgtcctacattatgaagccaaggacagagagacggctgtggtccagttttttgtga
ctggcagttaatcagagtcctttgctaggtagggtcctatatcttgtgtttctctacaacatatatgtgactttgaaatc
ctggaattcgtccaccccctgtcctactttagtgagacacaaggtacacctctaatgtcctcccttgttgccttagagta
gttaactttgaggacagaaaaaagcatagccagaagattgtaactgaaccgtcaactgttctgcccttggaacatgccta
ctttaagcacacgtagctttttgtgttgggaagtcaactgtatggatacttttctgttgacaaagtagccaaagacaatc
tgcagaaagtgttttctgcataataaaggcaatatatagcacctgg
求助 哪为高手帮我设计一下以下序列引物 要求 长度大于等于20bp 上游引物最好在1－100 下游1449－2046 不需要酶切位点 谢谢ggctccgcagagtgaccctttttcttggaaaagcggtggcgagagaagtgaaggtggctgttgggtagggagtcaagact
cctggaaggtggagagggtggcgggaggatgagctcgccgggcacagagagcgcagggaagagcctgcagtaccgagtgg
accacctgctcagcgccgtggagagcgagctgcaggcgggcagcgagaagggagaccccaccgaacgcgaactgcgagtg
ggcctggaggagagcgagctgtggctgcgcttcaaggagctaactaacgagatgattgtgaccaagaacggcaggaggat
gttcccggtgctgaaggtaaatgtgtcaggcctggaccccaatgccatgtactctttcttgctggacttcgtgacggctg
acaaccaccgctggaaatatgtgaacggggagtgggtacctgggggcaaaccagagcctcaggcgcccagctgcgtctac
atccacccagactcgcccaattttggggcccactggatgaaggcgcctgtgtctttcagcaaagtcaaactcaccaacaa
gctcaatggagggggacagatcatgttaaactccttgcataagtatgaacctcggattcacatcgtgagagttgggggcc
cgcaacgcatgatcaccagccactgctttcccgagacccagttcatagctgtgactgcctaccagaatgaggagattaca
gcccttaaaattaaatacaacccatttgctaaagccttccttgatgccaaagaaagaaacgaccacaaagatgtaatgga
ggaaccgggggactgccagcagccggggtattcccaatgggggtggcttgttcctggtgctggcaccctctgcccgcctg
ccagctcccaccctcagtttggaggctcgctctctctcccctccacacacggctgtgagaggtacccagctctaaggaac
caccggtcatcgccctaccccagcccctatgctcatcggaacagctctccaacctatgcggacaattcatctgcttgtct
gtccatgctgcagtcccatgataactggtctagcctcggagtgcctggccacaccagcatgctgcctgtgagtcataacg
ccagcccacctactggctctagccagtatcccagtctctggtctgtgagcaatggtaccatcaccccaggctcccagaca
gctggggtgtccaacgggctgggagctcagttctttcgaggctcccctgcacattacacaccactgacgcacacggtctc
agctgccacgtcctcgtcttctggttctccgatgtatgaaggggctgctacagtcacagacatttctgacagccagtatg
acacggcccaaagcctcctcatagcctcgtggacacctgtgtcacccccatctatgtgaattgaactttcctccatgtgc
tgagacttgtaacaaccggtgtcaactggatcttctaggctcaaagtggcaggctcttgggacaagggaaaaataaataa
ataaaagctagatactaacaactccattttcaaataagagcaataatacatgtcctataatcatgttctacagcctcttg
tttgatacctacagtagtgatatgtgtcctacattatgaagccaaggacagagagacggctgtggtccagttttttgtga
ctggcagttaatcagagtcctttgctaggtagggtcctatatcttgtgtttctctacaacatatatgtgactttgaaatc
ctggaattcgtccaccccctgtcctactttagtgagacacaaggtacacctctaatgtcctcccttgttgccttagagta
gttaactttgaggacagaaaaaagcatagccagaagattgtaactgaaccgtcaactgttctgcccttggaacatgccta
ctttaagcacacgtagctttttgtgttgggaagtcaactgtatggatacttttctgttgacaaagtagccaaagacaatc
tgcagaaagtgttttctgcataataaaggcaatatatagcacctgg
