• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        About LacZ,IPTG and X-Gal

        互联网

        1909

        载体操作中最常用的几个概念:

        1. The lac Z gene may be used as a simple "reporter" gene, separated from its natural promoter. For example, we might put the lac Z gene in front of a mammalian promoter, transfect the DNA into mammalian cells, and then fix and stain a tissue with X-gal. The cells that turn blue must have the beta galactosidase enzyme, the product of the lac Z gene, which could only have been expressed from the donated promoter. Ergo - the promoter was active. Actually, there are controls that are needed to make this brave statement, but you get the idea. We used only the coding sequence for the bacterial enzyme as a reporter, and it has nothing to do with the lac promoter in this scenario.

        2. We may use the lac promoter to drive or regulate expression of any gene in an appropriate prokaryotic cell (e.g. E. coli, where it comes from). That is, lac Z may be nowhere in the picture. We've just taken the regulatory machinery and are using it without the downstream genes.

        (责任编辑:大汉昆仑王)
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序