- ￥205
- 源叶
- 中国
- T90127-1ml
- 2025年10月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-80℃（运输条件：冰袋低温运输）
- 英文名：
IPTG (isopropyl-β-D-thiogalactopyranoside)
- 库存：
99
- 供应商：
上海源叶生物科技有限公司
- CAS号：
367-93-1
- 规格：
1ml
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文献和实验were ligated directionally to corresponding digested bacterial expession vector, pGEX2T (4.9 kb) after phosphatase treatment of the vector with calf intestine alkaline phosphatase. Transformation of ligated products was performed by using Top 10 (Invitrogen
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
411400 (Boehringer). For 10mM stock, weigh out 1mg (light sensitive!), add 27ul DMSO to solubilize, then add 12ul 3M NaOAc, pH5, mix, then 275ul H2O, Don''t make up large amountrs as this is not very stable, even at -80C. Diluted luciferin solution
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
T-3383) 12.5mg/ml 1.25g QS to 100ml with dH2 O, filter sterilize, aliquot 1ml, store at -20°C, lightsensitive ! Expression Library Titration : Streak the XL1-Blue MRF
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