- 询价
- Miltenyi Biotec
- 德国
- 130-094-538
- 2025年12月19日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
2−8°C
- 英文名：
Myeloid-Derived Suppressor Cell Isolation Kit, mouse
- 库存：
大量
- 供应商：
德国美天旎生物技术有限公司
概述
骨髓来源抑制性细胞(Myeloid-derived suppressor cells, MDSCs)是一群高度异质性的非成熟髓样细胞，具有单核样(monocytic- MDSCs或 M-MDSCs)和多型核样（polymorphonuclear-MDSCs或PMN-MDSCs）两大亚群，并对获得免疫及固有免疫发挥抑制功能，仅在病理条件下大量扩增。下游应用
该试剂盒用于从小鼠脾脏、淋巴组织或实体肿瘤组织内对Gr-1highLy-6G+和 Gr-1dimLy-6G–髓样细胞的分离和富集。应用举例
Human PBMCs were stained using the MDSC Detection Kit, human and analyzed by flow cytometry using the MACSQuant® Analyzer 10. A) To exclude red blood cells and identify leukocytes, a first gate on CD45+ cells was set (region R1). Next, to eliminate doublets, a gate on single cells in Forward scatter-A (A=area) versus Forward scatter-H (H=height) was set (region R2). These cells were further distinguished from debris via Forward scatter-A and Side scatter-A (region R3). Afterwards, a gate on viable cells (7-AAD– cells) was set (region R4). B) Cells from region R4 were further separated into 3 subsets: side scatterhigh (SSChigh) cells, which correspond to low density PMN-MDSCs (region R5), CD14+cells (region R6) and SSClowCD14– cells (region R7). Low density PMN-MDSCs contained in region R5 were further separated into 4 subsets based on the expression of CD16 and CD11b (gates R8 to R11). C) Cells stained with MDSC Control Cocktail from R6 were displayed with CD14 versus REA Control-FITC, and a region enclosing >99.5% of cells was set (region R12). This region was transferred to the cells labeled with MDSC Staining Cocktail, depicting M-MDSCs as CD14+HLA-DR–. D) Cells stained with MDSC Control Cocktail from region R7 were displayed with REA Control-FITC versus REA-Control-PE, and a region above the background staining, containing –CD33int.
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https://www.miltenyibiotec.com/DE-en/products/mdsc-detection-kit-human.html#130-126-233
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