The REAlease CD3 (TIL) MicroBead Kit has been developed for the isolation of CD3+ tumor-infiltrating leukocytes (TILs) from single-cell suspensions of solid human tumors as well as xenotransplanted tumors of mice with a humanized immune system. CD3 is expressed on T cells and their subsets as well as on natural killer T (NKT) cells. The REAlease CD3 (TIL) MicroBead Kit is an indirect magnetic labeling system that allows to obtain cells free of MicroBeads and the REAlease Biotin Complex.
下游应用
Isolation of specific CD3+ tumor-infiltrating T cell subsets. The selected CD3+ T cell population can be sorted according to a second marker of interest, for example:
CD56 for natural killer T (NKT) cells or γ/δ T cells by depleting TCR α/β T cells.
Isolation of CD3+ tumor-infiltrating T cells which need to be label-free.