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AffiniPure Goat Anti-Rabbit Ig

G H&L, HRP conjugated(bs-40295G-HRP)-100ul/1ml
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  • ¥360 - 2400
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  • bs-40295G-HRP
  • 2025年10月10日
  • 产品信息以Bioss网站为准
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    • 规格

      100ul/1ml

    规格:100ul产品价格:¥360.0
    规格:1ml产品价格:¥2400.0
    产品编号bs-40295G-HRP
    英文名称AffiniPure Goat Anti-Rabbit IgG H&L, HRP conjugated
    中文名称辣根过氧化物酶标记亲和纯化羊抗兔IgG (H+L)(进口分装)
    英文别名AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot)/HRP; AffiniPure Goat Anti-Rabbit IgG H&L/HRP; AffiniPure Goat Anti-Rabbit IgG H&L(HRP); HRP AffiniPure Goat Anti-Rabbit IgG (H+L); Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)
    中文别名HRP标记山羊抗兔IgG (H+L); 辣根过氧化物酶标记羊抗兔IgG (H+L)(进口分装);
    产品应用WB=1:5000-20000, IHC-P=1:3000-5000, IHC-F=1:3000-5000, ELISA=1:5000-20000

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Rabbit
    抗体来源Goat
    免疫原Native Rabbit IgG
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein G
    克隆类型Polyclonal
    浓度1.0 mg/ml
    储存液10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol.
    保存条件Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料HRP Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L), Highly Cross AdsorbedHRP antibodies can be used for a variety of applications, including ELISA, Western blotting, In-Cell Western assays, In-Gel Westerns, and many others.Isolation of specific antibodise was accomplished by immunoaffinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis, this antibody reacts with the heavy chains of rabbit IgG as well as the light chains common to most rabbit immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid phase adsorbed for minimal cross-reactivity with human, mouse, and rat serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and ELISA applications.

     

    应用推荐稀释比例
    {WB}{1:5000-20000}
    {IHC-P}{1:3000-5000}
    {IHC-F}{1:3000-5000}
    {ELISA}{1:5000-20000}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated (bs-5960R) at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (bs-40295G-HRP ) at 1/200 dilution and DAB staining.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated (bs-5960R) at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (bs-40295G-HRP ) at 1/200 dilution and DAB staining.
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated (bs-5960R) at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (bs-40295G-HRP ) at 1/200 dilution and DAB staining.
    产品细节图片4
    25 ug total protein per lane of various lysates (see on figure) probed with beta-Actin polyclonal antibody, unconjugated (bs-0061R) at 1:10000 dilution and 4°C overnight incubation.Followed by conjugated secondary antibody (bs-40295G-HRP) at 1:20000 dilution and r.t. for 60 min incubation.

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    图标文献和实验
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    [IF={{ 9.6 }}] {Yanan Fu. et al. Copper Peroxide-Ring Stabilized Bicelle Loaded with GSH-Responsive Derivative of Doxorubicin to Induce Amplified Cuproptosis. ADV HEALTHC MATER. 2025 May;:2500691} {} {}

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    [IF={{ 9 }}] {Yufeng Zhang. et al. An ancient regulatory variant of ACSF3 influences the coevolution of increased human height and basal metabolic rate via metabolic homeostasis. Cell Genomics. 2025 五月 21} {WB} {Human}

    [IF={{ 8.7 }}] {Hui Zeng. et al. Recombinant humanized type III collagen inhibits ovarian cancer and induces protective anti-tumor immunity by regulating autophagy through GSTP1. MATER TODAY BIO. 2024 Sep;:101220} {WB} {Human}

    [IF={{ 8.101 }}] {Shuang Xie. et al. Indispensable role of melatonin, a scavenger of reactive oxygen species (ROS), in the protective effect of Akkermansia muciniphila in cadmium-induced intestinal mucosal damage. FREE RADICAL BIO MED. 2022 Nov;193:447} {WB} {Mouse}

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    文献支持
    AffiniPure Goat Anti-Rabbit IgG H&L, HRP conjugated(bs-40295G-HRP)-100ul/1ml
    ¥360 - 2400