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AffiniPure Goat Anti-Rabbit Ig

G H&L, HRP conjugated(bs-40295G-HRP)-100ul/1ml

价格￥360 - 2400

品牌Bioss
货号bs-40295G-HRP
更新日期2025年10月10日

应用范围产品信息以Bioss网站为准

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应用范围：
产品信息以Bioss网站为准
规格：
100ul/1ml

规格：	100ul	产品价格：	￥360.0
规格：	1ml	产品价格：	￥2400.0

产品编号	bs-40295G-HRP
英文名称	AffiniPure Goat Anti-Rabbit IgG H&L, HRP conjugated
中文名称	辣根过氧化物酶标记亲和纯化羊抗兔IgG (H+L)(进口分装)
英文别名	AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot)/HRP; AffiniPure Goat Anti-Rabbit IgG H&L/HRP; AffiniPure Goat Anti-Rabbit IgG H&L(HRP); HRP AffiniPure Goat Anti-Rabbit IgG (H+L); Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)
中文别名	HRP标记山羊抗兔IgG (H+L); 辣根过氧化物酶标记羊抗兔IgG (H+L)(进口分装);
产品应用	WB=1:5000-20000, IHC-P=1:3000-5000, IHC-F=1:3000-5000, ELISA=1:5000-20000 Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
交叉反应	Rabbit
抗体来源	Goat
免疫原	Native Rabbit IgG
亚型	IgG
性状	Liquid
纯化方法	affinity purified by Protein G
克隆类型	Polyclonal
浓度	1.0 mg/ml
储存液	10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol.
保存条件	Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
背景资料	HRP Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L), Highly Cross AdsorbedHRP antibodies can be used for a variety of applications, including ELISA, Western blotting, In-Cell Western assays, In-Gel Westerns, and many others.Isolation of specific antibodise was accomplished by immunoaffinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis, this antibody reacts with the heavy chains of rabbit IgG as well as the light chains common to most rabbit immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid phase adsorbed for minimal cross-reactivity with human, mouse, and rat serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and ELISA applications.

应用	推荐稀释比例
{WB}	{1:5000-20000}
{IHC-P}	{1:3000-5000}
{IHC-F}	{1:3000-5000}
{ELISA}	{1:5000-20000}

P‌‌araformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated (bs-5960R) at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (bs-40295G-HRP ) at 1/200 dilution and DAB staining.

P‌‌araformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated (bs-5960R) at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (bs-40295G-HRP ) at 1/200 dilution and DAB staining.

P‌‌araformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated (bs-5960R) at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (bs-40295G-HRP ) at 1/200 dilution and DAB staining.

25 ug total protein per lane of various lysates (see on figure) probed with beta-Actin polyclonal antibody, unconjugated (bs-0061R) at 1:10000 dilution and 4°C overnight incubation.Followed by conjugated secondary antibody (bs-40295G-HRP) at 1:20000 dilution and r.t. for 60 min incubation.

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