重组人 ACE2 蛋白(His标签)(HPLC验证)

人 ACE2蛋白|ACE2 protein|ACE2(Human, C-His)（产品说明）

蛋白名称：ACE2蛋白, ACE2 protein

蛋白构建：A DNA sequence encoding the human ACE2 (NP_068576.1) (Met1-Ser740) was expressed with a polyhistidine tag at the C-terminus.

表达宿主：HEK293 Cells

蛋白纯度：> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.

蛋白活性：1. Measured by its binding ability in a functional ELISA. Immobilized 2019-nCoV Spike Protein (RBD, mFc Tag)(Cat: 40592-V05H) at 2 μg/mL (100 μL/well) can bind human ACE2 protein (His tag), the EC50 of ACE2 is 10-30 ng/mL. 2. Measured by its ability to cleave a fluorogenic peptide substrate, McaYVADAPK (Dnp)OH, R&D Systems, Catalog # ES007. The specific activity is >1000 pmols/min/μg.

蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.

预测N端：Gln 18

蛋白分子量：The recombinant human ACE2 consists of 734 amino acids and predicts a molecular mass of 85.1 kDa.

蛋白NP号：NP_068576.1

蛋白氨基酸序列：Met1-Ser740

蛋白标签：C-His

蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

缓冲液
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hardcopy of datasheet.

运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

复溶
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

ACE2 背景信息

Angiotensin-converting enzyme 2 (ACE2), a first homolog of ACE, regulates the renin angiotensin system (RAS) by counterbalancing ACE activity. Accumulating evidence in recent years has demonstrated a physiological and pathological role of ACE2 in the cardiovascular, renal and respiratory systems. ACE2 also has an important role in blood pressure control. This enzyme, an homolog of ACE, hydrolyzes angiotensin (Ang) I to produce Ang-(1-9), which is subsequently converted into Ang-(1-7) by a neutral endopeptidase and ACE. ACE2 releases Ang-(1-7) more efficiently than its catalysis of Ang-(1-9) by cleavage of Pro(7)-Phe(8) bound in Ang II. Thus, the major biologically active product of ACE2 is Ang-(1-7), which is considered to be a beneficial peptide of the RAS cascade in the cardiovascular system. A physiological role for ACE2 has been implicated in hypertension, cardiac function, heart function and diabetes. In the acute respiratory distress syndrome (ARDS), ACE, AngII, and AT1R promote the disease pathogenesis, whereas ACE2 and the AT2R protect from ARDS. Importantly, ACE2 has been identified as a key SARS-coronavirus receptor and plays a protective role in severe acute respiratory syndrome (SARS) pathogenesis. Furthermore, the recent explosion of research into the ACE2 homolog, collectrin, has revealed a new physiological function of ACE2 as an amino acid transporter, which explains the pathogenic role of gene mutations in Hartnup disorder. This review summarizes and discusses the recently unveiled roles for ACE2 in disease pathogenesis.