重组人 CD16a / FCGR3A 蛋白 (176 Val, His标签)(产品说明)
蛋白名称:Human CD16a / FCGR3A Protein (176 Val, His Tag)
蛋白构建:A DNA sequence encoding the extracellular domain (Met1-Gln208) of human CD16a (P08637-1, with natural variant Phe 176 Val) was expressed with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 97 % as determined by SDS-PAGE
蛋白活性:1. Using the Octet RED System, the affinity constant (Kd) of CD16a bound to Human IgG1 was 80 nM.2. Immobilized Human CD16a His (Cat: 10389-H08H1) at 2 μg/ml (100 μl/well) can bind Human IgG1 (Cat: 10702-HNAC), the EC50 of Human IgG1 is 150-450 ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Gly 17
蛋白分子量:The secreted recombinant human CD16a consists of 203 amino acids and predicts a molecular mass of 23.3 KDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of human CD16a is approximately 44.2 KDa due to glycosylation.
蛋白NP号:P08637-1
蛋白氨基酸序列:Met1-Gln208,F176V
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
北京义翘神州科技股份有限公司(简称:义翘神州,品牌:Sino Biological)于2021年8月16日成功登陆深圳证券交易所创业板(股票代码:301047),是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司,主要业务包括重组蛋白、抗体、基因和培养基等产品,以及重组蛋白、抗体的开发和生物分析检测等服务。
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