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- 细胞形态：
其他
- 生长状态：
悬浮生长
- 相关疾病：
其他疾病
- ATCC Number：
CRL-2959™
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 年限：
43 years
- 细胞类型：
其他细胞类型
- 组织来源：
peritoneal effusion
- 运输方式：
冻存运输
- 库存：
大量
| Designations: | SU-DHL-6 | ||
| Depositors: | AL Epstein | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast-like |
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| Source: | Tissue: peritoneal effusion Disease: large cell lymphoma; diffuse mixed histiocytic and lymphocytic lymphoma; follicular B cell lymphoma Derived from metastatic site: peritoneal cavity Cell Type: B lymphocyte |
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| Cellular Products: | monoclonal cytoplasmic immunoglobulin: IgM; lambda light chain | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1977 | ||
| Applications: | All of the DHL cell lines were negative for the presence of Epstein-Barr virus (EBV) genomes. SU-DHL-6 possesses a t(14;18)(q32;q21) translocation and demonstrates an unexpected recombination within its heavy chain gene locus that may be the interchromosomal breakpoint. They are composed of monoclonal populations of immunoglobin-bearing cells. |
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| DNA Profile (STR): | D5S818: 12 D13S317: 12,14 D7S820: 10 D16S539: 11,12 vWA: 14,17 THO1: 6,9.3 TPOX: 11,12 CSF1PO: 10 Amelogenin: X |
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| Cytogenetic Analysis: | This is a pseudodiploid cell line with a modal chromosome number of 47, a polyploidy rate of approximately 9% and one copy of the X chromosome. No Y chromosome was observed in any of the analyzed cells. A derivative t(14;18)(q32;q21) chromosome was present in most of the examined cells. Other derivative chromosomes were generally consistent with previous studies, these include: del(6)(p23), del(9)(p21.1?), add(11)(q25), i(17q) [or t(6?;17)(p10;q10)?] and del(18)(q21)?. [PubMed: 3881165]In addition, there was an add(7)(q22?), an add(21p?) and 4-7 unidentified marker chromosomes.Note: Cytogenetic information is based on initial seed stock at ATCC . Cytogenetic instability has been reported in the literature for some cell lines. [16173194 ] | ||
| Age: | 43 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | The DHL cell lines were successfully established in continuous suspension culture from 10 patients with a histopathological diagnosis of diffuse histiocytic lymphoma (DHL). They are composed of monoclonal populations of immunoglobin-bearing cells. All of the DHL cell lines were negative for the presence of Epstein-Barr virus (EBV) genomes. SU-DHL-6 possesses a t(14;18)(q32;q21) translocation and demonstrates an unexpected recombination within its heavy chain gene locus that may be the interchromosomal breakpoint. In SU-DHL-6, the t(14;18) translocation juxtaposes a truncated bcl-2 gene with J6 in a tail-to-head configuration. The deregulated expression of the altered bcl-2 gene may play a critical role in the disordered growth and differentiation of follicular B cell lymphoma. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Protocol: Cultures can be maintained by the addition of fresh medium. An inoculum of 8 X 10(4) to 3 x 10(5) cells/ml is recommended. Subculture when cell concentration is between 7 x 10(5) and 1 x 10(6) cells/ml. Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended. Medium renewal: Every 3 to 4 days |
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| Preservation: | Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO liquid nitrogen vapor phase |
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| Doubling Time: | approximately 29 hours | ||
| Related Products: | Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2001 Recommended serum: ATCC 30-2020 Cell culture tested DMSO: ATCC 4-X Erythrosin B vital stain solution: ATCC 30-2404 |
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| References: | 16173192: Hua C, et al. Consequences of the t(14;18) chromosomal translocation in follicular lymphoma: deregulated expression of a chimeric and mutated BCL-2 gene. Oncogene Res. 2(3): 263-275, 1988. PubMed: 3285301 16173193: Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220 16173194: Kaiser-McCaw Hecht B, et al. Histiocytic lymphoma cell lines: immunologic and cytogenetic studies. Cancer Genet. Cytogenet. 14 (3-4): 205-218, 1985. PubMed: 3881165 16173195: Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039 16173198: Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794 16173199: Bakhshi A, et al. Cloning the chromosomal breakpoint of t(14;18) human lymphomas: clustering around JH on chromosome 14 and near a transcriptional unit on 18. Cell. 41(3):899-906, 1985. PubMed: 3924412 |
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SU-DHL-6
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