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- 生长状态：
悬浮生长
- 运输方式：
冻存运输
- 库存：
大量
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- ATCC Number：
HB-8709™
- 免疫类型：
IgG3
- 细胞形态：
淋巴样
| Designations: | KC-4G3 [KC-4(IgG3)] | ||
| Depositors: | Coulter Corporation | ||
| Isotype: | IgG3 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against human carcinoma cells | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with prostatic adenocarcinoma cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with an antigen present on solid tumors (98 of 104 tested) and cross-reacts with minimal cross reaction with paraffin embedded (14 of 92) or frozen (1 of 33) normal tissues. The antigen is found in the cytoplasm as a 490000 dalton molecule, and in the membrane and cytoplasm as a 438000 dalton molecule. An antibody with the same specificity but of the IgM class is available (ATCC HB-8710 ). |
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| Propagation: | ATCC complete growth medium: Modified Dulbecco's medium with 0.1 mM hypoxanthine and 0.015 mM thymidine; fetal bovine serum, 15% |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | fetal bovine serum, 90%; DMSO, 10% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X | ||
| References: | 3910: Kortright KH, Hofheinz DE. Monoclonal antibody to a human carcinoma tumor associated antigen. US Patent 4,708,930 dated Nov 24 1987 | ||
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠CXCL1/KC (KC)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液、唾液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗小鼠 KC 单抗包被于酶标板上，标准品和样品中的 KC与单抗结合，加入生物素化的抗小鼠 KC
，那么我们从这一张图，就能判断ChIP测序是否成功，钱花得值不值。 更有意思的是，我们在多个H3K4me3的ChIP测序项目中都观察到了相似Peak分布图：TSS附近出现非常明显的结合峰，长度约2kb。 （项目KC2018-CXX，动物组蛋白H3K4me3） （项目KC2018-CXX，动物组蛋白H3K4me3） （项目KC2018-CXX，植物组蛋白H3K4me3） （项目KC2018-CXX，植物组蛋白H3K4me3） 这与文献中
如何判断 ChIP 抗体是否结合目的蛋白，完美的完成了任务？ChIP 后进行 WB 检测就尤为重要了，检测报告能告诉我们 ChIP 实验结果究竟如何，WB 检测不合格文章可能被 argue 哦。ChIP 实验报告怎么看？我们以几个项目的检测报告为例给大家解释 ChIP 的实验结果。项目编号：KC- 2018 -C**。这个项目是对小鼠的细胞进行组蛋白 ChIP，使用组蛋白特异性抗体，ChIP 后的 WB 检测结果是这样的：首先，这是一个非常好的结果：除 marker 外共有 9 个泳道
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