- 询价
- 2025年11月22日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 器官来源：
胚胎
- ATCC Number：
CRL-2206™
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 年限：
embryo
- 生长状态：
贴壁生长
- 品系：
NIH/Swiss
- 运输方式：
冻存运输
- 库存：
大量
- 细胞形态：
成纤维样
| Designations: | PA317 LXSN 6E6 | ||
| Depositors: | DA Galloway | ||
| Biosafety Level: | 2 [Cells contain human papilloma viral DNA sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | fibroblast |
||
| Source: | Organ: embryo Strain: NIH/Swiss |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | Note: The user of the PA317 LXSN 6E6 cell line agrees to indemnify and hold harmless the United States, the ATCC , Denise A. Galloway and Fred Hutchinson Cancer Research Center, Seattle, Washington from any claims, costs, damages, or expenses resulting from any injury (including death), damage or loss that may arise from the use of the cell line either directly (including use for diagnostic purposes) or in the preparation of a product. The user assumes all risks and responsibilities in connection with the receipt, handling, storage and use of the material. | ||
| Age: | embryo | ||
| Comments: | PA317 LXSN 6E6 is a packaging cell line developed by transfection of the retrovirus vector pLXSN6bE6 into the Psi-2 ecotropic packaging cell line. Virions produced from the transfected Psi-2 cells were used to infect the amphotropic packaging line PA317, and infected cells were selected in medium containing G418. The pLXSN6bE6 vector contains the human papilloma virus (HPV) type 6b E6 gene under control of the Moloney murine leukemia virus (MoMuLV) promoter - enhancer sequences. The vector also contains a gene controlling resistance to neomycin transcribed from the SV40 promoter. This line produces the amphotropic retrovirus LXSN6bE6 which encodes the HPV6b E6 open reading frame, and which can be used to stably infect many cell types. The resulting cells constitutively express the E6 protein of HPV6b. |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
||
| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended Medium Renewal: Every 2 to 3 days Remove medium, rinse flask with fresh 0.25% trypsin, 0.02% EDTA and remove trypsin, Add an additional 1 to 2 ml of trypsin solution, and allow the flask to sit at room temperature (or 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
||
| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| References: | 22566: Halbert CL, et al. The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. J. Virol. 65: 473-478, 1991. PubMed: 1845902 | ||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验的分枝尖端，形成帽状间充质，促进帽状间充质中的肾祖细胞增殖分化，形成聚集(Pre-tubular aggregate，PA)。随后，PA 和 UB 分别进行间充质-上皮细胞转化（Mesenchymal-epithelial transition, MET）和小管生成，进而形成肾单位【2】。目前广泛用于肾脏研究的人肾脏细胞系忽略了细胞外微环境，与在体的器官类型和整体特性仍存在较大差异，不能充分体现出肾脏的作用及其特点，而三维培养的肾类器官模型为肾脏的研究提供了新途径。人源多能干细胞衍生的肾脏类器官
【原创】自己总结的RNAi干预的癌基因(75条) 大家一起来补全好吗
Adenocarcinoma (Hela cells) Hec1 Chromosomal segregation Her2/neu #3 HPV E6 (143) HPV E6/E7 H-Ras (138) H-ras (wild-type and V12 mutant) IGF-1R K-Ras (58) K-ras (V12) mutant livin Antiapoptotic Adenocarcinoma Livin/ML-IAP/KIAP(212) MAPK #3 Matrix
中国医学科学院药物研究所再帕尔•阿不力孜团队：一种高灵敏、高覆盖质谱成像技术新进展
在肾髓质的特异性分布，进一步说明 AFADESI-MSI 的高灵敏度扩大了的检测范围。 图 2 | 硫苷脂（STs）在大鼠脑和肾中的提取离子色谱（EIC）轮廓和 MSI 较宽动态范围有助于提供精确的代谢产物分布。应用非靶向 AFADESI-MSI 方法在对功能代谢物的检测中实现了超过三个数量级的动态范围。图 3 显示甜菜碱及其同位素离子的离子峰强度分别为 1.2E6，6.7E4，5.1E3 和 1.1E3，并显示出清晰一致的髓质特异性分布。 图 3 | 大鼠肾脏中甜菜碱及其同位素离子的 MSI
技术资料暂无技术资料 索取技术资料
