- ￥1350 - 3000
- Proteintech
- 66520-1-Ig
- 2025年10月13日
- WB, IHC, IF/ICC, FC (Intra), IP, ELISA
- Mouse
- human, mouse, rat
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human PARP1 protein
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
- 靶点：
PARP1
- 抗体英文名：
PARP1 Monoclonal antibody
- 抗体名：
PARP1 Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | Jurkat cells, RAW 264.7 cells, HeLa cells, HSC-T6 cells, HepG2 cells, ROS1728 cells, NIH/3T3 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human lung cancer tissue, mouse colon tissue, mouse testis tissue, human breast cancer tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Neuro-2a cells |
| Positive FC (Intra) detected in | HeLa cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66520-1-Ig targets PARP1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, chicken, zebrafish |
| 免疫原 |
CatNo: Ag19173 Product name: Recombinant human PARP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-327 aa of BC037545 Sequence: MAESSDKLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGHSIRHPDVEVDGFSELRWDDQQKVKKTAEAGGVTGKGQDGIGSKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQVRLSKKMVDPEKPQLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQT |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | poly (ADP-ribose) polymerase 1 |
| 别名 | ARTD1, ADPRT1, ADPRT 1, ADPRT, ADP-ribosyltransferase diphtheria toxin-like 1 |
| 计算分子量 | 1014 aa, 113 kDa |
| 观测分子量 | 113-116 kDa, 85-89 kDa |
| GenBank蛋白编号 | BC037545 |
| 基因名称 | PARP1 |
| Gene ID (NCBI) | 142 |
| RRID | AB_2881883 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P09874 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes, and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and give rise to fragments ranging from 42-89-kDa. This antibody was generated against the N-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Sci Adv Arginine methylation of BRD4 by PRMT2/4 governs transcription and DNA repairAuthors - Liu Liu |
| human | WB | Nat Chem Biol Enhanced mapping of small-molecule binding sites in cellsAuthors - Jacob M Wozniak |
| rat | WB,IP | Nat Commun Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.Authors - Megha Jhanji |
| human | IHC | J Exp Clin Cancer Res PARP1-targeted fluorescence molecular endoscopy as novel tool for early detection of esophageal dysplasia and adenocarcinomaAuthors - Sabrina Marcazzan |
| human | WB | Biomaterials Urinary exosomes-based Engineered Nanovectors for Homologously Targeted Chemo-Chemodynamic Prostate Cancer Therapy via abrogating IGFR/AKT/NF-kB/IkB signaling.Authors - Shaojun Pan |
| human | WB | Arthritis Rheumatol Association of the Polymorphism rs13259960 in SLEAR With Predisposition to Systemic Lupus Erythematosus.Authors - Zhen Fan |
抗体的检测外，还应该用与其抗原成分相关的其它抗原进行交叉试验，方法可用ELISA、IFA法。例如：①制备抗黑色素瘤细胞的McAb，除用黑色素瘤细胞反应外，还应该用其它脏器的肿瘤细胞和正常细胞进行交叉反应，以便挑选肿瘤特异性或肿瘤相关抗原的单克隆抗体。②制备抗重组的细胞因子的单克隆抗体，应首先考虑是否与表达菌株的蛋白有交叉反应，其次是与其它细胞因子间有无交叉。 2．McAb的Ig类与亚类的鉴定 一般在用酶标或荧光素标记的第二抗体进行筛选时已经基本上确定了抗体的Ig类型
免疫系统受抗原刺激后，B细胞转化为浆细胞，由浆细胞产生与抗原发生特异性结合的球蛋白，这类球蛋白称为抗体(antibody,Ab)。 免疫球蛋白(immunoglobulim,Ig)是指具有抗体活性或化学结构与抗体相似的球蛋白。 免疫球蛋白普遍存在于哺乳动物和人类的血液、组织液和外分泌中。人体的免疫球蛋白主要有五类：IgM、IgA、IgG、IgD、IgE。 (一) 抗体结构 抗体是具有4条多肽链的对称
浓度很高。可见该法操作简便、经济，不过，腹水中常混有小鼠的各种杂蛋白（包括Ig），因此在很多情况下要提纯后才能使用，而且还有污染动物病毒的危险，故而最好用SPF级小鼠。（1）材料：a. 成年BALB/c小鼠。b. 降植烷（Pristane）或液体石蜡。c. 处于对数生长期的杂交瘤细胞。（2）方法：a. 腹腔接种降植烷或液体石蜡，每只小鼠0.3-0.5ml。b. 7-10天后腹腔接种用PBS或无血清培养基稀释的杂交瘤细胞，每只小鼠5×105/0.2ml。c. 间隔5天后，每天观察小鼠腹水产生情况，如腹部
技术资料暂无技术资料 索取技术资料
