- ￥1350 - 3000
- Proteintech
- 55184-1-AP
- 2025年10月13日
- WB, IHC, ELISA
- Rabbit
- human, mouse
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- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, ELISA
- 靶点：
WNT5A/B
- 抗体英文名：
WNT5A/B Polyclonal antibody
- 抗体名：
WNT5A/B Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | mouse heart tissue, HUVEC cells, PC-3 cells |
| Positive IHC detected in | human lung cancer tissue, human thyroid cancer tissue, human tonsillitis tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55184-1-AP targets WNT5A/B in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, RIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, chicken, zebrafish |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | wingless-type MMTV integration site family, member 5A |
| 别名 | WNT5A, hWNT5A, Protein Wnt 5a, Protein Wnt-5a, WNT5A B |
| 计算分子量 | 42 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | NM_003392 |
| 基因名称 | WNT5A |
| Gene ID (NCBI) | 7474 |
| RRID | AB_2881285 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P41221 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathway. Wnt5A is a member of the Wnt family of proteins, which are 38-45 kDa secreted cysteine-rich proteins with hydrophobic signal peptides. This antibody can recognize WNT5A and WNT5B.
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文献和实验AP Assay and FGF Binding Mike Naski COS-7 cells were transfected by a standard DEAE-dextran method with 4 mg of plasmid/10 6 cells. Conditioned medium was harvested 3, 6 and 9 days after transfection and used for AP enzyme
Using ProHits to Store, Annotate, and Analyze Affinity Purification–Mass Spectrometry (AP‐MS) Data
address. (C ) Standard Web browser on the host computer (here, Mozilla Firefox on Mac OS X). ProHits Lite VM may be accessed by typing the IP address from panel B into the host computer browser. Other computers on the network can access the VM
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
600, then dilute the cells to final OD600 of 0.5, place on ice. Screening Plaques with AP-Fusion Protein Reagents: 1M IPTG stock solution NZY plates with phage 20 x
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