- ￥1350 - 3000
- Proteintech
- 51067-2-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
- Rabbit
- human, mouse, rat, pig
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- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat, pig
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
- 靶点：
Beta Catenin
- 抗体英文名：
Beta Catenin Polyclonal antibody
- 抗体名：
Beta Catenin Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, HEK-293T cells, NIH/3T3 cells, C6 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | mouse liver tissue, human colon cancer tissue, human liver cancer tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue, mouse epididymis tissue |
| Positive IF/ICC detected in | MCF-7 cells, HepG2 cells, HeLa cells, T-47D cells, mouse testis tissue |
| Positive FC (Intra) detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
51067-2-AP targets Beta Catenin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, chIP |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human, mouse, rat, pig, canine, chicken, zebrafish, hamster, sheep, goat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | catenin (cadherin-associated protein), beta 1, 88kDa |
| 别名 | b cat, B-catenin, beta-Catenin, Catenin beta 1, Catenin beta-1 |
| 计算分子量 | 781 aa, 86 kDa |
| 观测分子量 | 92 kDa |
| GenBank蛋白编号 | BC058926 |
| 基因名称 | Beta Catenin |
| Gene ID (NCBI) | 1499 |
| ENSEMBL Gene ID | ENSG00000168036 |
| RRID | AB_2086128 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35222 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)
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- 作者
- 内容
- 询问日期
文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB,IF,IP,IHC | Cell Metab Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.Authors - Xiuying Zhong |
| human | IF | Mol Cancer PMID: 32727463Authors - Cefan Zhou |
| human | WB | Hepatology Basolateral CD147 induces hepatocyte polarity loss by E-cadherin ubiquitination and degradation in hepatocellular carcinoma progress.Authors - Meng Lu |
| mouse,rat | WB | Acta Pharm Sin B Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.Authors - Xunxun Wu |
| mouse | WB | Hepatology Long noncoding RNA DANCR increases stemness features of hepatocellular carcinoma via de-repression of CTNNB1.Authors - Sheng-Xian Yuan |
| zebrafish | IF | Nat Commun ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling.Authors - Xiaoyun Sun |
liqiuling 怎么才能获取beta catenin的基因？ chuckdouble pubmed里面有Nucleotide.到那里找. liqiuling 不太会看，而且合成的话，很贵，想知道如果想获得别人的惠赠，该怎样做？哪位大侠支个招？不胜感激！ lmnsmu 在NCBI的核酸库中找到其序列，然后设计引物，通过RT-PCR扩增获得该基因
familar 小妹困惑，在此请教大师！ 做了几次beta-catenin的表达（western），都没做出来，目的蛋白的分子量是92，可是，做出来的印记总是在下方，明显的不是目的蛋白的位置。我师兄说，做beta-catenin要提核蛋白才能做出来，可我之前也有做出来过的！请教各位有经验的同志，这样的现象是何解？ woxingwosu 不一定要提核蛋白才能出来。b-catenin在核内核外都有分布。应该
【求助】细胞里的beta catenin 好检测吗？？ 用 western blot
antonio2001 细胞里的beta catenin 好检测吗？？ 用 western blot 和免疫细胞化学 ？？ 2008851029 细胞里的beta-actin非常好检测（我用的是ST的抗体） littlebee83325 什么细胞呢？胞浆蛋白么？ magichunter beta catenin 好检测，细胞全蛋白裂解液上样20uL（15
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