ACC1 多克隆抗体 21923-1-AP产品图
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ACC1 多克隆抗体 21923-1-AP

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  • ¥1350 - 3000
  • Proteintech已认证
  • 21923-1-AP
  • 2025年10月13日
  • WB, IHC, IF/ICC, IP, ELISA
  • Rabbit
  • human, mouse, rat
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    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 免疫原

      Recombinant human ACC protein

    • 亚型

      IgG

    • 保存条件

      Store at -20°C. Stable for one year after shipment.

    • 克隆性

      Polyclonal

    • 标记物

      Unconjugated

    • 适应物种

      human, mouse, rat

    • 保质期

      详见产品包装

    • 库存

      充足

    • 宿主

      Rabbit

    • 应用范围

      WB, IHC, IF/ICC, IP, ELISA

    • 靶点

      ACC1

    • 抗体英文名

      ACC1 Polyclonal antibody

    • 抗体名

      ACC1 Polyclonal antibody

    • 规格

      50ul/100ul/150ul

    规格:50ul产品价格:¥1350.0
    规格:100ul产品价格:¥2200.0
    规格:150ul产品价格:¥3000.0

    经过测试的应用

    Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, mouse brain tissue, rat brain tissue
    Positive IP detected inHepG2 cells
    Positive IHC detected inmouse skeletal muscle tissue, mouse brain tissue
    Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
    Positive IF/ICC detected inHeLa cells
    Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

    推荐稀释比

    应用推荐稀释比
    Western Blot (WB)WB : 1:1000-1:20000
    Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
    Immunohistochemistry (IHC)IHC : 1:50-1:500
    Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
    It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
    Sample-dependent, Check data in validation data gallery.

    产品信息

    21923-1-AP targets ACC1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

    经测试应用 WB, IHC, IF/ICC, IP, ELISA
    文献引用应用WB, IHC, IF, IP, RIP
    经测试反应性 human, mouse, rat
    文献引用反应性human, mouse, rat, pig, chicken, bovine
    免疫原

    CatNo: Ag16452

    Product name: Recombinant human ACC protein

    Source: e coli.-derived, PGEX-4T

    Tag: GST

    Domain: 2260-2383 aa of BC137287

    Sequence: LLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLAEWLEKQLTEEDGVHSVIEENIKCISRDYVLKQIRSLVQANPEVAMDSIIHMTQHISPTQRAEVIRILSTMDSPST

    宿主/亚型 Rabbit / IgG
    抗体类别 Polyclonal
    产品类型 Antibody
    全称 acetyl-Coenzyme A carboxylase alpha
    别名 ACACA, ACAC, ACC, ACCA, ACC-alpha
    计算分子量 2383 aa, 275 kDa
    观测分子量 250 kDa
    GenBank蛋白编号BC137287
    基因名称 Acetyl-CoA Carboxylase 1
    Gene ID (NCBI) 31
    RRIDAB_11042445
    偶联类型 Unconjugated
    形式Liquid
    纯化方式Antigen affinity purification
    UNIPROT IDQ13085
    储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
    储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

    背景介绍

    ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.

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    • 作者
    • 内容
    • 询问日期
    图标文献和实验
    该产品被引用文献
    [1]Kaili sour soup in alleviation of hepatic steatosis in rats via lycopene route: an experimental study.
    PMID:40257305Ann Med2025-12
    [2]Pharmacological induction of acetyl-CoA carboxylase 1 autophagic degradation attenuates lipid accumulation and cholangiocarcinoma progression
    PMID:41291880J Exp Clin Cancer Res2025-11
    [3]FOXK2 regulates fatty acid metabolism and promotes cervical cancer progression by activating the mTOR/DRP1 signaling axis.
    PMID:40641601Front Cell Dev Biol2025-06-26
    [4]Lipid synthesis, triggered by PPARγ T166 dephosphorylation, sustains reparative function of macrophages during tissue repair.
    PMID:39179603Nat Commun2024-08-23
    [5]Hepatic regulator of G protein signaling 14 ameliorates NAFLD through activating cAMP-AMPK signaling by targeting Giα1/3.
    PMID:38237897Mol Metab2024-02
    [6]17β-Estradiol (E2) Upregulates the ERα/SIRT1/PGC-1α Signaling Pathway and Protects Mitochondrial Function to Prevent Bilateral Oophorectomy (OVX)-Induced Nonalcoholic Fatty Liver Disease (NAFLD).
    PMID:38136219Antioxidants (Basel)2023-12-12
    [7]STK25 enhances hepatocellular carcinoma progression through the STRN/AMPK/ACC1 pathway.
    PMID:34986838Cancer Cell Int2022-01-05
    [8]The Mutual Inhibition of FoxO1 and SREBP-1c Regulated the Progression of Hepatoblastoma by Regulating Fatty Acid Metabolism.
    PMID:34539243Mediators Inflamm2021-09-08
    [9]Dlgap1 negatively regulates browning of white fat cells through effects on cell proliferation and apoptosis.
    PMID:32169116Lipids Health Dis2020-03-13
    [10]Deletion of OSBPL2 in auditory cells increases cholesterol biosynthesis and drives reactive oxygen species production by inhibiting AMPK activity.
    PMID:31427568Cell Death Dis2019-08
    相关实验
    • 有适合免疫沉淀IP的GST抗体吗?

      因此,综合来说,EarthOx的GST标签抗体 (货号#E022040)是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时,我们也提供其他品牌的GST标签抗体,如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody(货号#10000-0-AP)以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体,请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞

    • 抗体选择

      实验,请根据说明书列出已验证的种属来选择适合你实验的抗体。   (4)样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体,待测样本蛋白的结构域和样本在提取和处理过程中是否会变性,蛋白空间构象的改变,会影响抗体的免疫亲和反应。   (5)单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主,一般单克隆抗体特异性强,但亲和力相对小,检测抗原灵敏度相对就低;而多克隆抗体特异性稍弱,但抗体的亲和力强,灵敏度高,但易出现非特异性染色(可以通过封闭等避免

    • 石蜡切片免疫组织化学染色

      发生,最好在特异性抗体处理切片之前,选择与二抗种属相同的非免疫血清封闭电荷,阻止一抗与之结合,抑制非特异性背景着色。常用方法是用2%~10%羊血清或2%~5%牛血清白蛋白在室温下作用10~30分钟。但应注意此种结合不牢固,所以最好不要冲洗,倾去余液直接加一抗。SABC或SP免疫组织化学试剂盒中常配有非免疫血清。 7.第一抗体孵育 滴加特异性一抗于切片上,4℃孵育24~48小时,或室温孵育过夜,也可在37~C孵育1~2小时。之后,用PBS冲洗3次,每次5分钟。一抗分为多克隆抗体和单克

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