- ￥1350 - 3000
- Proteintech
- 11554-1-AP
- 2025年10月13日
- WB, IHC, FC (Intra), IP, ELISA
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Cyclin E protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, FC (Intra), IP, ELISA
- 靶点：
Cyclin E1
- 抗体英文名：
Cyclin E1 Polyclonal antibody
- 抗体名：
Cyclin E1 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HT-29 cells, NIH/3T3 cells, mouse heart tissue, HeLa cells, Jurkat cells, human lung tissue, MCF-7 cells, HepG2 cells, K-562 cells, human placenta tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse testis tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HeLa cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11554-1-AP targets Cyclin E1 in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, pig, zebrafish, artemia sinica |
| 免疫原 |
CatNo: Ag2110 Product name: Recombinant human Cyclin E protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 111-410 aa of BC035498 Sequence: ANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cyclin E1 |
| 别名 | CCNE1, G1/S-specific cyclin-E1, G1/S specific cyclin E1, Cyclin E, CCNE |
| 计算分子量 | 410 aa, 47 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC035498 |
| 基因名称 | Cyclin E1 |
| Gene ID (NCBI) | 898 |
| RRID | AB_2071066 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P24864 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis. Two bands of Cyclin E1 were expressed in U2OS and MDA-MB-231 cells (PMID:9858585, PMID: 24112607).
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | Nat Commun Foxk1 and Foxk2 promote cardiomyocyte proliferation and heart regenerationAuthors - Dongcheng Cai |
| human | WB | Mol Cancer LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.Authors - Xi Jin |
| human | WB | Adv Sci (Weinh) Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate CancerAuthors - Rong Wang |
| mouse | IHC | Theranostics Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproductionAuthors - Meng Gao |
| human | WB | Nat Commun Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma.Authors - Dan Su |
| mouse | WB | Nat Commun Iron-dependent histone 3 lysine 9 demethylation controls B cell proliferation and humoral immune responses.Authors - Yuhang Jiang |
microRNA-195通过直接影响靶基因Cyclin D1和Cyclin E1的表达 抑制了人神经胶质瘤细胞的增殖
膜母细胞瘤（pRb）和增殖细胞核抗原（PCNA）的形成。相反，抑制了miR-195的作用后，神经胶质瘤细胞的增殖速度加快，S期细胞的比例升高，G1/G0期的细胞数减少，提高细胞的非依赖型锚定生长能力，促进细胞中pRb和PCNA的磷酸化。此外，我们还发现miR-195通过直接作用于Cyclin D1和Cyclin E1的mRNA 3’UTR区下调Cyclin D1’和Cyclin E1的表达，抑制了神经胶质瘤细胞的增殖。综上所述，该实验结果表明miR-195在抑制神经胶质瘤细胞增殖的过程中发挥了重要的作用，并阐明
-1 (31) 978-998 AATTGGTGCACTTTTACGTGA IMR90E1 ARC21
的抑癌作用。他克隆了 Rb 全长的 cDNA ，发现其产物是一种长 928 氨基酸，分子量为 105Kda 的蛋白质。 Rb 的分子功能是什么呢？它主要是可以和其它各种蛋白相互作用，其中包括肿瘤抗原： SV40 T 抗原，腺病毒 E1A 抗原，人类乳头瘤病毒 E7 抗原，可结合到 RB 的 379~792 残基区域。一种可能是部分致癌蛋白由于和 RB 产物的相互作用其致癌的作用受到了抑制。另外 RB 还有抑制细胞增殖的作用。
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