- ￥1350 - 3000
- Proteintech
- 16806-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human IL-1 beta protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 靶点：
IL-1 beta
- 抗体英文名：
IL-1 beta Polyclonal antibody
- 抗体名：
IL-1 beta Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | LPS and Protein transport inhibitor treated THP-1 cells, human adrenal gland tissue, A431 cells |
| Positive IHC detected in | human tonsillitis tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | LPS treated THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16806-1-AP targets IL-1 beta in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 文献引用应用 | WB, IHC, IF, ELISA |
| 经测试反应性 | human |
| 文献引用反应性 | human, pig, rabbit, canine, zebrafish, bovine, sheep, duck |
| 免疫原 |
CatNo: Ag10295 Product name: Recombinant human IL-1 beta protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-269 aa of BC008678 Sequence: MAEVPELASEMMAYYSGNEDDLFFEADGPKQMKCSFQDLDLCPLDGGIQLRISDHHYSKGFRQAASVVVAMDKLRKMLVPCPQTFQENDLSTFFPFIFEEEPIFFDTWDNEAYVHDAPVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 1, beta |
| 别名 | Catabolin, IL 1, IL 1 beta, IL1 beta, IL1- beta |
| 计算分子量 | 269 aa, 31 kDa |
| 观测分子量 | 30-35 kDa |
| GenBank蛋白编号 | BC008678 |
| 基因名称 | IL1B |
| Gene ID (NCBI) | 3553 |
| ENSEMBL Gene ID | ENSG00000125538 |
| RRID | AB_10646432 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P01584 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Immunity Omega-3 fatty acids prevent inflammation and metabolic disorder through inhibition of NLRP3 inflammasome activation.Authors - Yiqing Yan |
| zebrafish | IF | Cell Migratory Neural Crest Cells Phagocytose Dead Cells in the Developing Nervous System.Authors - Yunlu Zhu |
| WB | Trends Biotechnol Chairside live biotherapeutic hydrogel for comprehensive periodontitis therapyAuthors - Kaifeng Li | |
| WB,IF | Small M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting InflammationAuthors - Jie Sun | |
| zebrafish | IF | Nat Commun Dynamic control of proinflammatory cytokines Il-1β and Tnf-α by macrophages in zebrafish spinal cord regeneration.Authors - Themistoklis M Tsarouchas |
Nat Commun Downregulation of cytoplasmic DNases is implicated in cytoplasmic DNA accumulation and SASP in senescent cells.Authors - Akiko Takahashi |
加入不同稀释度的IL-1α或IL-1β，100μl/孔， 同时设1％FCS RPMI 1640对照 ↓ 5％CO,37℃培养18～24小时 ↓ 按终稀释度为1∶10～20转入另一块96孔板中
panzerking 我想用IL-1beta 刺激HepG2细胞 用NF-kB-luc报告基因来检测nfkb的激活 想请教刺激前是否要用无血清的培养液饥饿细胞 或者是将IL-1溶解在PBS里 用PBS来刺激细胞 细胞是否不能培养在5%的血清培养液中呢 请战友指点 谢谢 螺旋 转染NF-kB-luc报告基因24小时以后,换成无血清的培养基继续培养2-10个小时,需要看你的细胞
2. 抗原与填料的连接 这一步是整个纯化过程中最关键的一步，如果在这一步上有什么失误，建议你从step one重新开始吧。 首先要注意一点：常见的连接方法都基于抗原上的游离氨基或者巯基与活化后的beads的连接，因此体系中不应该有其它含有氨基或巯基的化合物，包括尿素、硫脲、盐酸胍、Tris、铵根离子、Beta-巯基乙醇等，如果有这些物质在体系中，应该首先设法除去。 溴化氰活化的琼脂糖与氨基化合物反应的机理如下： 环氧氯丙烷活化的琼脂糖与氨基化合物反应
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