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- 询价记录
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human p38 MAPK protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Rabbit
- 应用范围:
WB, IHC, ELISA
- 靶点:
p38 MAPK
- 抗体英文名:
p38 MAPK Polyclonal antibody
- 抗体名:
p38 MAPK Polyclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, mouse heart tissue, Jurkat cells, RAW 264.7 cells, Neuro-2a cells, NIH/3T3 cells, rat spleen tissue, rat heart tissue, K-562 cells |
| Positive IHC detected in | human colon cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14064-1-AP targets p38 MAPK in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, rabbit, chicken, zebrafish, goat, fish, duck |
| 免疫原 |
CatNo: Ag5115 Product name: Recombinant human p38 MAPK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-307 aa of BC031574 Sequence: MSQERPTFYRQELNKTIWEVPERYQNLSPVGSGAYGSVCAAFDTKTGLRVAVKKLSRPFQSIIHAKRTYRELRLLKHMKHENVIGLLDVFTPARSLEEFNDVYLVTHLMGADLNNIVKCQKLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDCELKILDFGLARHTDDEMTGYVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLTGRTLFPGTDHIDQLKLILRLVGTPGAELLKKISSESARNYIQSLTQMPKMNFANVFIGANPLAVDLLEKMLVLDSDKRITAAQALAHAY |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mitogen-activated protein kinase 14 |
| 别名 | MAPK14, Cytokine suppressive anti-inflammatory drug-binding protein, CSPB1, CSBP2, CSBP1 |
| 计算分子量 | 360 aa, 41 kDa |
| 观测分子量 | 38-42 kDa |
| GenBank蛋白编号 | BC031574 |
| 基因名称 | p38 MAPK |
| Gene ID (NCBI) | 1432 |
| RRID | AB_2878007 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16539 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cellself-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has some isoforms with MW 29-31 kDa, 35 kDa and 41 kDa.
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文献和实验【求助】请问如何检测RAS、MAPK、p38、p21 ?参与者:liqunfeng请问如何检测RAS、MAPK、p38、p21??谁帮帮我啊参与者:xiaolx你在网上搜搜别人的实验中怎么检测的,不就知道了。p38,我师姐做过免疫组化。参与者:zhangyuelin1999检测信号通路现在用的比较多的方法应该是Western blot,它是测细胞中的蛋白含量(半定量)的最好方法了,免疫组化检测是定性的!如果时间充裕的话,你可以两种方法都可以做的。RAS:Ras蛋白是细胞内的一种小分子GTP结合
ampk细胞通路是通过磷酸化激活下游的,磷酸化的就是活化形式啦,比如c-jun吧,有钱的话还是要要买c-jun和p-c-jun的,紧张的话但用p-c-jun也能说明问题,里面的各型无非就是多抗,兔或鼠单抗,兔单抗比较好,选择的话还要看你做什么用途,个人浅见 ampk? 还是mapk? 如果是AMPK的话, 那么离AP-1还很远, 最起码中间还隔着MAPK. :D:D 如果LZ想看表达的话不买Total c-jun
yy59410 在下在做MAPK通路对皮肤成纤维细胞凋亡诱导的作用,我分别用p38,JNK,ERK相应的通路抑制剂阻断相应的通路后看给药后细胞的情况,发现这些通路的阻断并不能阻断药物对细胞的凋亡诱导,想请教坛子里的高手们这是怎么回事?我接下来需要做什么? 希望大侠们赐教。不甚感激啊。 chuckdouble yy59410 wrote: 在下在做MAPK通路对皮肤成纤维
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