- ￥900 - 1900
- Proteintech
- PE-65047
- 2025年10月13日
- FC
- Hamster
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
PE Fluorescent Dye
- 适应物种：
Mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Hamster
- 应用范围：
FC
- 靶点：
CD103
- 抗体英文名：
PE Anti-Mouse CD103 (2E7)
- 抗体名：
PE Anti-Mouse CD103 (2E7)
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥900.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥1900.0 |
经过测试的应用
| Positive FC detected in | mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
PE-65047 targets CD103 in FC applications and shows reactivity with Mouse samples.
| 经测试应用 | FC |
| 经测试反应性 | Mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Hamster / IgG |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | integrin alpha E, epithelial-associated |
| 别名 | alpha E1, alpha M290, aM290, CD103, Integrin alpha E, Integrin alpha M290, Itgae |
| GenBank蛋白编号 | BC150690 |
| 基因名称 | CD103 |
| Gene ID (NCBI) | 16407 |
| RRID | AB_2883847 |
| 偶联类型 | PE Fluorescent Dye |
| 最大激发/发射波长 | 496 nm, 565 nm / 578 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | Q60677 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD103, also known as integrin alpha-E (ITGAE) or integrin alpha-IEL, is a type I transmembrane integrin protein that binds integrin beta 7 to form Integrin alpha-E beta-7 which is a receptor for E-cadherin and mediates adhesion of T lymphocytes to epithelial cells (PMID: 7969453; 8468482). CD103 is expressed on intraepithelial lymphocyte (IEL) T cells (both alpha/beta T cells and gamma/delta T cells), some peripheral regulatory T cells (Tregs), lamina propria T cells, and a subset of dendritic cells in the gut mucosa and mesenteric lymph nodes (PMID: 12242333; 15608520; 16216890).
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Isolation of Human and Mouse Neutrophils Ex Vivo and In Vitro
; Wartha and Henriques-Normark, Sci Signal 1:pe25, 2008). Neutrophils are, therefore, always a major focus of investigation for scientists all over the world. Isolation of neutrophils from either human beings or animals such as mice is a very common
Phycoerythrin conjugation protocol
and Centricon concentrators. Add 0.5% sodium azide. Store at 4 °C protected from light. Do not freeze. Figure 1. Analysis of column eluate fractions from PE conjugation of monoclonal antibody IB4 (murine IgG2a, anti-human CD
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