- ￥1350 - 3000
- Proteintech
- 13423-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IP, ELISA
- Rabbit
- human, mouse
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Caspase 8 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IP, ELISA
- 靶点：
Caspase 8/P43/P18
- 抗体英文名：
Caspase 8/P43/P18 Polyclonal antibody
- 抗体名：
Caspase 8/P43/P18 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | Staurosporine treated Jurkat cells, UV treated Hela, RAW264.7 |
| Positive IP detected in | HeLa cells, Jurkat cells |
| Positive IHC detected in | human lymphoma tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13423-1-AP targets Caspase 8/P43/P18 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, RIP, ELISA |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, pig, rabbit, chicken, zebrafish, hamster, sheep |
| 免疫原 |
CatNo: Ag4214 Product name: Recombinant human Caspase 8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-270 aa of BC028223 Sequence: MDFSRNLYDIGEQLDSEDLASLKFLSLDYIPQRKQEPIKDALMLFQRLQEKRMLEESNLSFLKELLFRINRLDLLITYLNTRKEEMERELQTPGRAQISAYRVMLYQISEEVSRSELRSFKFLLQEEISKCKLDDDMNLLDIFIEMEKRVILGEGKLDILKRVCAQINKSLLKIINDYEEFSKERSSSLEGSPDEFSNGEELCGVMTISDSPREQDSESQTLDKVYQMKSKPRGYCLIINNHNFAKAREKVPKLHSIRDRNGTHLDAGTV |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | caspase 8, apoptosis-related cysteine peptidase |
| 别名 | CASP8, Caspase 8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4 |
| 计算分子量 | 538 aa, 62 kDa |
| 观测分子量 | 53-57 kDa, 32-45 kDa |
| GenBank蛋白编号 | BC028223 |
| 基因名称 | Caspase 8 |
| Gene ID (NCBI) | 841 |
| RRID | AB_2068463 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14790 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.
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- 作者
- 内容
- 询问日期
文献和实验| Species | Application | Title |
|---|---|---|
| rat | WB | Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse modelAuthors - Mengyan Hu |
| IP | Sci Adv HSPA13 facilitates NF-κB-mediated transcription and attenuates cell death responses in TNFα signaling.Authors - Chun Gao | |
| human | WB | Exp Mol Med PRKCSH contributes to TNFSF resistance by extending IGF1R half-life and activation in lung cancerAuthors - Gu-Choul Shin |
| human | WB | Nat Commun Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms.Authors - Christoph Grohmann |
| human | WB | Aging Dis MiR-29a-3p Improves Acute Lung Injury by Reducing Alveolar Epithelial Cell PANoptosis.Authors - Yanhui Cui |
| human | WB | EMBO J A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.Authors - Xu Wang |
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