- ￥1350 - 3000
- Proteintech
- 10176-2-AP
- 2025年10月13日
- WB, IHC, IF-P, IF-Fro, IP, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human AKT protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF-P, IF-Fro, IP, ELISA
- 靶点：
AKT
- 抗体英文名：
AKT Polyclonal antibody
- 抗体名：
AKT Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells, C6 cells, C2C12 cells, mouse brain tissue, rat brain tissue, Jurkat cells, RAW 264, HSC-T6 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF-Fro detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10176-2-AP targets AKT in WB, IHC, IF-P, IF-Fro, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, IF-Fro, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ELISA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, monkey, chicken, zebrafish, sheep, goat, fish, zebra finches |
| 免疫原 |
CatNo: Ag0213 Product name: Recombinant human AKT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-224 aa of BC000479 Sequence: MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLC |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | v-akt murine thymoma viral oncogene homolog 1 |
| 别名 | AKT 1, AKT1, EC:2.7.11.1, PKB, PKB ALPHA |
| 计算分子量 | 56 kDa |
| 观测分子量 | 56-62 kDa |
| GenBank蛋白编号 | BC000479 |
| 基因名称 | AKT1 |
| Gene ID (NCBI) | 207 |
| RRID | AB_2224574 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P31749 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
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- 作者
- 内容
- 询问日期
文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Cell Res RAB31 marks and controls an ESCRT-independent exosome pathway.Authors - Denghui Wei |
| mouse | WB | Nat Cell Biol Heat stress activates YAP/TAZ to induce the heat shock transcriptome.Authors - Min Luo |
| human | WB | Nat Commun Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer riskAuthors - Pingting Ying |
| human | WB | Adv Sci (Weinh) Cis-Regulation of an m6A Eraser by an Insertion Variant Associated with Survival of Patients With Non-Small Cell Lung CarcinomaAuthors - Lei Cheng |
| human | WB | Sci Adv Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes.Authors - Patrick Han |
| mouse,human | WB | Acta Pharm Sin B Dissecting the novel abilities of aripiprazole: The generation of anti-colorectal cancer effects by targeting Gαq via HTR2BAuthors - Haowei Liu |
signaling, #9272, 不能识别磷酸化AKT，只能识别非磷酸化AKT。 所以，最好用磷酸化抗体来做。 liubingood 非磷酸化的多克隆抗体（比如兔多抗）一般是可以识别磷酸化蛋白的，但是通常磷酸化蛋白与非磷酸化蛋白的分子量没有什么区别，所以做出的带子通常是非磷酸化和磷酸化蛋白叠加的带子，而且磷酸化蛋白只占非磷酸化蛋白的很小很小一部分，我们就认为那是由非磷酸化蛋白组成的。要想检测磷酸化蛋白，两种方法：特异的磷酸化抗体检测；采用免疫沉淀的方法先用非磷酸化抗体
祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
SAB(Signalway antibody ) 是一家汇聚了多名专家和学者的新型高科技生物公司，其研发和生产的抗体包括了数百种磷酸化 / 非磷酸化抗体 ( 可以提供同一位点的磷酸化 / 非磷酸化的成对抗体 ) ，为神经科学、细胞信号转导 (Akt pathway ， Jat/Sat pathway ， MAPK pathway ， NF-Kappa B pathway 等 ) 、癌症 / 凋亡、细胞周期、染色质 / 转录、细胞骨架通道、胰岛素 / 葡萄糖、核受体 / 膜受体
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