- ￥1350 - 3000
- Proteintech
- 10375-2-AP
- 2025年10月13日
- WB, IHC, IF/ICC, FC (Intra), ELISA
- Rabbit
- human
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human MMP9 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, FC (Intra), ELISA
- 靶点：
MMP-9 (N-terminal)
- 抗体英文名：
MMP-9 (N-terminal) Polyclonal antibody
- 抗体名：
MMP-9 (N-terminal) Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | Jurkat cells, HEK-293 cells, LNCaP cells, HepG2 cells, Raji cells, MDA-MB-231 cells |
| Positive IHC detected in | human breast cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10375-2-AP targets MMP-9 (N-terminal) in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA |
| 文献引用应用 | WB, IHC, IF, CoIP, ELISA |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, pig, rabbit, bovine, hamster, fish |
| 免疫原 |
CatNo: Ag0552 Product name: Recombinant human MMP9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-100 aa of BC006093 Sequence: MSLWQPLVLVLLVLGCCFAAPRQRQSTLVLFPGDLRTNLTDRQLAEEYLYRYGYTRVAEMRGESKSLGPALLLLQKQLSLPETGELDSATLKAMRTPRCG |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) |
| 别名 | MMP9 (N-terminal), MMP9, Matrix metalloproteinase 9, GELB, EC:3.4.24.35 |
| 计算分子量 | 707 aa, 78 kDa |
| 观测分子量 | 92 kDa, 67 kDa |
| GenBank蛋白编号 | BC006093 |
| 基因名称 | MMP-9 |
| Gene ID (NCBI) | 4318 |
| RRID | AB_10897178 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P14780 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92 kDa gelatinase, 92 kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades collagens type IV and V. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Gastroenterology Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.Authors - Haojie Jin |
| mouse | WB,IHC | Cell Host Microbe Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formationAuthors - Zhenyu Tian |
| human | WB | Mol Cancer CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.Authors - Bing Yao |
| mouse | WB | Adv Sci (Weinh) Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease ProgressionAuthors - Rui Zhou |
| human | WB, IHC | J Pineal Res Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.Authors - Li Cui |
| mouse | IF | Acta Pharm Sin B Engineering cannabidiol synergistic carbon monoxide nanocomplexes to enhance cancer therapy via excessive autophagyAuthors - Chang Xiao |
Assessment of Gelatinases (MMP-2 and MMP-9 by Gelatin Zymography
are multidomain proteases that contain a zinc atom in the active site and are produced in a latent inactive form (zymogen) (2 ). Acquisition of enzymatic activity requires cleavage of the inhibitory N-terminal domain (3 ). Thus, generation of the active form
人基质金属蛋白酶-9（MMP-9）酶联免疫分析（ELISA）
人基质金属蛋白酶 -9 （ MMP-9 ） 酶联免疫分析（ ELISA ） 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中 基质金属蛋白酶-9 （MMP-9 ） 含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中 人基质金属蛋白酶 9 （ MMP-9 ） 水平。用纯化的 人基质金属蛋白酶 9 （ MMP-9 ） 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中
相关专题 生物帮之抗体制备 技术服务 详细描述 服务标准 SC1050 抗肽多克隆抗体制备(金斯瑞合成抗原 ) 服务内容 交付 a.多肽合成 （少于15个氨基酸，纯度大于85%，10mg） b.C末段或N末段
技术资料暂无技术资料 索取技术资料
