- ￥1080 - 3220
- Sino Biological
- 北京
- 13100-H08H
- 2025年08月12日
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- 详细信息
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- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human IGFBP7 / IBP-7 Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20.00 µg/50.00 µg
| 规格： | 20.00 µg | 产品价格： | ￥1080.0 |
|---|---|---|---|
| 规格： | 50.00 µg | 产品价格： | ￥3220.0 |
蛋白名称：Human IGFBP7 / IBP-7 Protein (His Tag)
蛋白构建：A DNA sequence encoding the human IGFBP7 (Q16270) (Met 1-Leu 282) was fused with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 92 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Ser 27
蛋白分子量：The secreted recombinant human IGFBP7 comprises 267 amino acids and has a predicted molecular mass of 27.9 kDa. Since IGFBP7 can be proteolytically cleaved between lysine 97 and alanine 98, the apparent molecular mass of rh IGFBP7 is approximately 36 & 32 kDa in SDS-PAGE under reducing conditions, corresponding to the whole protein and the cleaved form respectively.
蛋白NP号：Q16270
蛋白氨基酸序列：Met1-Leu282
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Chromatin Interaction Analysis Using Paired‐End Tag Sequencing
Analysis using Paired?End Tag sequencing (ChIA?PET) is a technique developed for large?scale, de novo analysis of higher?order chromatin structures. Cells are treated with formaldehyde to cross?link chromatin interactions, DNA segments bound by protein
.3 ). Concatamers of tags are cloned and sequenced to yield a STAGE library. Tags in the library represent DNA fragments that were occupied by the DNA?binding protein, and mapping these tag sequences to the genome identifies the binding loci of the DNA?binding
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
Basic Protocol 1: Generation of an Active Recombinant Protein That is End‐Labeled Basic Protocol 2: Hydroxyl Radical Cleavage of Protein in Absence and Presence of Ligand Basic Protocol 3: Analyze Gel
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