重组人 VEGFR2 / Flk-1 / CD309 / KDR 蛋白 (His 标签)

重组人 VEGFR2 / Flk-1 / CD309 / KDR 蛋白 (His标签)（产品说明）
蛋白名称：Human VEGFR2 / Flk-1 / CD309 / KDR Protein (His Tag)
蛋白构建：A DNA sequence encoding the extracellular domain of human VEGFR2 (NP_002244.1) (Met 1-Glu 764) was fused with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 97 % as determined by SDS-PAGE
蛋白活性：1. Using the Octet RED System, the affinity constant (Kd) of VEGFR2 / KDR Protein, Human, Recombinant (His Tag) (Cat. 10012-H08H) bound to Anti-VEGFR2 Antibody (Cat. 10012-MM03) was 1.9 μM. 2. Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 10-50μg/mL in the presence of 10 ng/mL recombinant human VEGF165.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Ala 20
蛋白分子量：The recombinant human VEGFR2 consists of 756 amino acids and predicts a molecular mass of 84.6 kDa. The apparent molecular mass of human VEGFR2 is approximatly 120-130 in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号：NP_002244.1
蛋白氨基酸序列：Met1-Glu764
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.