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- Sino Biological
- 北京
- 10605-HNAE
- 2026年08月31日
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- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Human EGF / Epidermal Growth Factor Protein
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20 µg/100 µg/500 µg/1 mg
| 规格： | 20 µg | 产品价格： | ￥169.0 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | ￥480.0 |
| 规格： | 500 µg | 产品价格： | ￥1080.0 |
| 规格： | 1 mg | 产品价格： | ￥1480.0 |
蛋白名称：重组人EGF / Epidermal Growth Factor蛋白
蛋白构建：A DNA sequence encoding the mature form of human EGF (NP_001954.2) (Asn971-Arg1023) was expressed and purified with an initial Met at the N-terminus.
表达宿主：E. coli
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
生物活性：1.iPSC-derived human vascular organoids (Day 7) were cultured with FGF2 (Cat#10014-HNAE), VEGFA (Cat# 11066-HNAH), EGF (Cat#10605-HNAE). Red arrows represent vascular organoids. Image taken at 10x magnification.(Routinely tested) 2.Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.02-0.2ng/ml. 3.Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with EGF (Cat # 10605-HNAE)(10 ng/mL, 30min) using Phospho-EGF Receptor (Tyr1068) rabbit monoclonal Antibody (Cat# 110463-R0027) at 1:2000 dilution (upper), or Anti-EGFR Antibody, Rabbit Polyclonal (Cat# 100372-T32) at 1:2000 dilution (lower)(Routinely tested).
内毒素：< 5 EU per mg of the protein.
预测N端：Met
蛋白分子量：The recombinant human EGF consisting of 54 amino acids and has a calculated molecular mass of 6.35 kDa as estimated in SDS-PAGE under reducing conditions.
蛋白NP号：NP_001954.2
蛋白氨基酸序列：Asn971-Arg1023
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文献和实验Regulator of Epidermal Growth Factor Signaling: Sprouty
Sprouty was first discovered through its downregulatory effect on fibroblast growth factor (FGF) receptor pathway during tracheal development. Sprouty expression is also induced by the epidermal growth factor receptor (EGFR) cascade
Epithelial Cell Migration in Response to Epidermal Growth Factor
Epidermal growth factor (EGF) is a ligand for the EGF receptor, a member of the erbB family of receptor tyrosine kinases. Activation of EGF receptor by EGF or other high-affinity ligands often results in increased migration of cells
Semiquantitative immunoblots of membrane protein-epidermal growth factor
Membrane proteins or cytokines are sometimes difficult to isolate and purify. Our group recently concentrated on epidermal growth factor (EGF) protein expression studies. Mature EGF was initially identified from mouse submaxillary gland extract
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