Hieff多片段一步法快速克隆试剂盒(重组克隆试剂盒)

[ 1 ]Deep-sea megafauna co-opts microbial energy metabolism genes to withstand ultra-long starvation

Journal:CELL|DOI:10.1016/j.cell.2026.05.012|IF:45.1

[ 2 ]Reversible phase separation of HSF1 is required for an acute transcriptional response during heat shock

Journal:NATURE CELL BIOLOGY|DOI:10.1038/s41556-022-00846-7|IF:28.21

[ 3 ]Immunogenetic losses co-occurred with seahorse male pregnancy and mutation in tlx1 accompanied functional asplenia

Journal:Nature Communications|DOI:10.1038/s41467-022-35338-7|IF:17.69

[ 4 ]Noncanonical amino acids as doubly bio-orthogonal handles for one-pot preparation of protein multiconjugates

Journal:Nature Communications|DOI:10.1038/s41467-023-36658-y|IF:16.6

[ 5 ]Cytoplasmic RNase III Drosha controls lipogenesis by noncanonical regulation of SREBP1

Journal:MOLECULAR CELL|DOI:10.1016/j.molcel.2026.03.028|IF:16

[ 6 ]Engineering a 660 nm-Responsive Optogenetic Inducer of Pyroptosis for Precision Cancer Therapy

Journal:Advanced Science|DOI:10.1002/advs.76768|IF:14.1

[ 7 ]Mitochondrial heterogeneity drives the evolution of fungicide resistance in Phytophthora sojae, with associated fitness trade-offs

Journal:Science Advances|DOI:10.1126/sciadv.adz4601|IF:13.9

[ 8 ]NF8: A miniature pentameric coiled-coil fusion tag for enhanced recombinant protein expression

Journal:CHEMICAL ENGINEERING JOURNAL|DOI:10.1016/j.cej.2026.175740|IF:12.5

[ 9 ]An efficient and multiple target transgenic RNAi technique with low toxicity in Drosophila

Journal:Nature Communications|DOI:10.1038/s41467-018-06537-y|IF:12.35

[ 10 ]CRISPR-dCas13-tracing reveals transcriptional memory and limited mRNA export in developing zebrafish embryos

Journal:GENOME BIOLOGY|DOI:10.1186/s13059-023-02848-6|IF:12.3

[ 11 ]Developing a highly efficient hydroxytyrosol whole-cell catalyst by de-bottlenecking rate-limiting steps

Journal:Nature Communications|DOI:10.1038/s41467-020-14918-5|IF:12.12

[ 12 ]Promiscuous enzymatic activity-aided multiple-pathway network design for metabolic flux rearrangement in hydroxytyrosol biosynthesis

Journal:Nature Communications|DOI:10.1038/s41467-019-08781-2|IF:11.88

[ 13 ]A single-component light sensor system allows highly tunable and direct activation of gene expression in bacterial cells

Journal:NUCLEIC ACIDS RESEARCH|DOI:10.1093/nar/gkaa044|IF:11.5

[ 14 ]Novel Mycorrhiza-Specific P Transporter PvPht1;6 Contributes to As Accumulation at the Symbiotic Interface of As-Hyperaccumulator Pteris vittata

Journal:ENVIRONMENTAL SCIENCE & TECHNOLOGY|DOI:10.1021/acs.est.2c04367|IF:11.36

[ 15 ]Sequential resolution of (S) and (R)-6-fluoro-chroman-2-carboxylic acid by two esterases in turn

Journal:GREEN CHEMISTRY|DOI:10.1039/D1GC04512A|IF:11.03

[ 16 ]Even allocation of benefits stabilizes microbial community engaged in metabolic division of labor

Journal:Cell Reports|DOI:10.1016/j.celrep.2022.111410|IF:10

[ 17 ]The LIN and LINL E3 ligases function redundantly in arbuscular mycorrhizal symbiosis and nodulation of Medicago truncatula

Journal:NEW PHYTOLOGIST|DOI:10.1111/nph.71052|IF:8.7

[ 18 ]Dye disaggregation light-up aptamer for super-resolution RNA imaging

Journal:SENSORS AND ACTUATORS B-CHEMICAL|DOI:10.1016/j.snb.2023.133731|IF:8.4

[ 19 ]Rapamycin targets STAT3 and impacts c-Myc to suppress tumor growth

Journal:Cell Chemical Biology|DOI:10.1016/j.chembiol.2021.10.006|IF:8.12

[ 20 ]Digital Counting of Breaks Labeling In Situ: A Fast and Absolute Quantification Method for Measurement of DNA Double-Strand Breaks Based on Digital Polymerase Chain Reaction

Journal:ANALYTICAL CHEMISTRY|DOI:10.1021/acs.analchem.2c03985|IF:8.01

[ 21 ]Dynamic control of toxic natural product biosynthesis by an artificial regulatory circuit

Journal:METABOLIC ENGINEERING|DOI:10.1016/j.ymben.2019.12.002|IF:7.81

[ 22 ]A monoclonal antibody targeting the C-terminal of α-synuclein fibrils mitigates pathology in a Parkinson’s disease model

Journal:Cell Reports|DOI:10.1016/j.celrep.2025.116897|IF:7.7

[ 23 ]Metabolically engineering of Yarrowia lipolytica for the biosynthesis of naringenin from a mixture of glucose and xylose

Journal:BIORESOURCE TECHNOLOGY|DOI:10.1016/j.biortech.2020.123726|IF:7.54

[ 24 ]Tumor Microenvironment-Responsive Antibody Fragment-Cleavable PEGylated Drug Conjugates (AFCDC) for Improved Tumor Penetration and Antitumor Efficacy

Journal:JOURNAL OF MEDICINAL CHEMISTRY|DOI:10.1021/acs.jmedchem.5c03215|IF:7.3

[ 25 ]A plug-and-play cytochrome P450 system enables de novo biosynthesis of 2-phenylethanol derivatives

Journal:METABOLIC ENGINEERING|DOI:10.1016/j.ymben.2026.102493|IF:7.3

[ 26 ]Novel crosstalk between ethylene- and jasmonic acid-pathway responses to a piercing–sucking insect in rice

Journal:NEW PHYTOLOGIST|DOI:10.1111/nph.16111|IF:7.3

[ 27 ]Growth-Coupled Screening Enables Efficient Engineering of Key Enzymes for Chlorogenic Acid Biosynthesis

Journal:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY|DOI:10.1021/acs.jafc.5c14119|IF:6.7

[ 28 ]The Medicago truncatula hydrolase MtCHIT5b degrades Nod factors of Sinorhizobium meliloti and cooperates with MtNFH1 to regulate the nodule symbiosis

Journal:Frontiers in Plant Science|DOI:10.3389/fpls.2022.1034230|IF:6.63

[ 29 ]Characterization of Rabbit Nucleotide-Binding Oligomerization Domain 1 (NOD1) and the Role of NOD1 Signaling Pathway during Bacterial Infection

Journal:Frontiers in Immunology|DOI:10.3389/fimmu.2017.01278|IF:6.43

[ 30 ]Engineering probiotic-derived outer membrane vesicles as functional vaccine carriers to enhance immunity against SARS-CoV-2

Journal:iScience|DOI:10.1016/j.isci.2022.105772|IF:6.11

[ 31 ]Biosynthesis of (S)-Equol from Soy Whey by Metabolically Engineered Escherichia coli

Journal:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY|DOI:10.1021/acs.jafc.3c00439|IF:6.1

[ 32 ]Effect of Hcp Iron Ion Regulation on the Interaction Between Acinetobacter baumannii With Human Pulmonary Alveolar Epithelial Cells and Biofilm Formation

Journal:Frontiers in Cellular and Infection Microbiology|DOI:10.3389/fcimb.2022.761604|IF:6.07

[ 33 ]A Flagella Hook Coding Gene flgE Positively Affects Biofilm Formation and Cereulide Production in Emetic Bacillus cereus.

Journal:Frontiers in Microbiology|DOI:10.3389/fmicb.2022.897836|IF:6.06

[ 34 ]Proteomining-Based Elucidation of Natural Product Biosynthetic Pathways in Streptomyces

Journal:Frontiers in Microbiology|DOI:10.3389/fmicb.2022.913756|IF:6.06

[ 35 ]Comparative transcriptomic analysis of Glycine soja and G. max and functional identification of GsCNGC20-d interacted with GsCDPK29 under salt stress

Journal:ENVIRONMENTAL AND EXPERIMENTAL BOTANY|DOI:10.1016/j.envexpbot.2022.105185|IF:6.03

[ 36 ]ROS-Responsive Bio-hybrid Probiotics for Synergistic Acute Lung Injury Therapy

Journal:COLLOIDS AND SURFACES B-BIOINTERFACES|DOI:10.1016/j.colsurfb.2026.115903|IF:5.9

[ 37 ]Glucosinolate Sulfatases–Sulfatase-Modifying Factors System Enables a Crucifer-Specialized Moth To Pre-detoxify Defensive Glucosinolate of the Host Plant

Journal:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY|DOI:10.1021/acs.jafc.2c03929|IF:5.9

[ 38 ]Characterization and Heterologous Expression of UDP-Glucose 4-Epimerase From a Hericium erinaceus Mutant with High Polysaccharide Production

Journal:Frontiers in Bioengineering and Biotechnology|DOI:10.3389/fbioe.2021.796278|IF:5.89

[ 39 ]Reconstruction of Secondary Metabolic Pathway to Synthesize Novel Metabolite in Saccharopolyspora erythraea

Journal:Frontiers in Bioengineering and Biotechnology|DOI:10.3389/fbioe.2021.628569|IF:5.89

[ 40 ]Wheat AGAMOUS LIKE 6 transcription factors function in stamen development by regulating the expression of Ta APETALA3

Journal:DEVELOPMENT|DOI:10.1242/dev.177527|IF:5.76

[ 41 ]Ethylene Promotes Expression of the Appressorium- and Pathogenicity-Related Genes via GPCR- and MAPK-Dependent Manners in Colletotrichum gloeosporioides

Journal:Journal of Fungi|DOI:10.3390/jof8060570|IF:5.72

[ 42 ]Cherry Valley Ducks Mitochondrial Antiviral-Signaling Protein-Mediated Signaling Pathway and Antiviral Activity Research

Journal:Frontiers in Immunology|DOI:10.3389/fimmu.2016.00377|IF:5.7

[ 43 ]The chromosome-scale genome assembly of Jasminum sambac var. unifoliatum provides insights into the formation of floral fragrance

Journal:Horticultural Plant Journal|DOI:10.1016/j.hpj.2023.03.003|IF:5.7

[ 44 ]Selenocysteine-Specific Mass Spectrometry Reveals Tissue-Distinct Selenoproteomes and Candidate Selenoproteins

Journal:Cell Chemical Biology|DOI:10.1016/j.chembiol.2018.08.006|IF:5.59

[ 45 ]A CRISPR-Cas9 Strategy for Activating the Saccharopolyspora erythraea Erythromycin Biosynthetic Gene Cluster with Knock-in Bidirectional Promoters

Journal:ACS Synthetic Biology|DOI:10.1021/acssynbio.9b00024|IF:5.57

[ 46 ]The transcription factor GmbHLH3 confers Cl−/salt tolerance to soybean by upregulating GmCLC1 expression for maintenance of anion homeostasis

Journal:ENVIRONMENTAL AND EXPERIMENTAL BOTANY|DOI:10.1016/j.envexpbot.2021.104755|IF:5.55

[ 47 ]Enterovirus D68 Infection Induces TDP-43 Cleavage, Aggregation, and Neurotoxicity

Journal:JOURNAL OF VIROLOGY|DOI:10.1128/jvi.00425-23|IF:5.4

[ 48 ]Combinatory Biosynthesis of Prenylated 4-Hydroxybenzoate Derivatives by Overexpression of the Substrate-Promiscuous Prenyltransferase XimB in Engineered E. coli

Journal:ACS Synthetic Biology|DOI:10.1021/acssynbio.8b00070|IF:5.32

[ 49 ]High GC Content Cas9-Mediated Genome-Editing and Biosynthetic Gene Cluster Activation in Saccharopolyspora erythraea

Journal:ACS Synthetic Biology|DOI:10.1021/acssynbio.7b00448|IF:5.32

[ 50 ]AcERF1B and AcERF073 Positively Regulate Indole-3-acetic Acid Degradation by Activating AcGH3.1 Transcription during Postharvest Kiwifruit Ripening

Journal:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY|DOI:10.1021/acs.jafc.1c03954|IF:5.28

[ 51 ]De Novo Biosynthesis of Chlorogenic Acid Using an Artificial Microbial Community

Journal:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY|DOI:10.1021/acs.jafc.0c07588|IF:5.28

[ 52 ]Secretory expression and purification of recombinant PLA2R epitopes for the detection of anti-PLA2R autoantibody in serum

Journal:ANALYST|DOI:10.1039/D2AN00094F|IF:5.23

[ 53 ]Building a Versatile Protein Production Platform Using Engineered Trichoderma reesei

Journal:ACS Synthetic Biology|DOI:10.1021/acssynbio.1c00570|IF:5.11

[ 54 ]CAMKIIγ is a targetable driver of multiple myeloma through CaMKIIγ/ Stat3 axis

Journal:Aging-US|DOI:10.18632/aging.103490|IF:4.83

[ 55 ]Efflux identification and engineering for ansamitocin P-3 production in Actinosynnema pretiosum

Journal:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY|DOI:10.1007/s00253-020-11044-6|IF:4.81

[ 56 ]Transposon insertion sequencing identifies novel genes involved in product synthesis in gas-fermenting Clostridium ljungdahlii

Journal:Synthetic and Systems Biotechnology|DOI:10.1016/j.synbio.2026.04.011|IF:4.8

[ 57 ]Engineering of multiple modules to enhance lignocellulose degradation ability in Bacillus subtilis using CRISPR/Cas9 system

Journal:Synthetic and Systems Biotechnology|DOI:10.1016/j.synbio.2026.01.021|IF:4.8

[ 58 ]Juvenile Hormone Membrane Signaling Enhances its Intracellular Signaling Through Phosphorylation of Met and Hsp83

Journal:Frontiers in Physiology|DOI:10.3389/fphys.2022.872889|IF:4.76

[ 59 ]Histone Acetyltransferase Rtt109 Regulates Development, Morphogenesis, and Citrinin Biosynthesis in Monascus purpureus

Journal:Journal of Fungi|DOI:10.3390/jof9050530|IF:4.7

[ 60 ]Multiplexed site-specific genome engineering in Mycolicibacterium neoaurum by Att/Int system

Journal:Synthetic and Systems Biotechnology|DOI:10.1016/j.synbio.2022.05.006|IF:4.69

[ 61 ]Repurposing screen using a robust human rhinovirus infectious clone identifies pyrvinium pamoate with antiviral activity

Journal:ANTIVIRAL RESEARCH|DOI:10.1016/j.antiviral.2026.106417|IF:4.3

[ 62 ]CRISPR/Cas9-mediated lgp2 knockout and cross-species rescue reveal the immunoregulatory role of LGP2 in zebrafish

Journal:FISH & SHELLFISH IMMUNOLOGY|DOI:10.1016/j.fsi.2026.111308|IF:4.2

[ 63 ]Engineering Yarrowia lipolytica for Enhanced Production of Arbutin

Journal:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY|DOI:10.1021/acs.jafc.9b07151|IF:4.19

[ 65 ]The SlPCL1–SlSUMO1 Complex Defines a SlPCL1–SlNPF4.6 Module Governs Cold Tolerance in Tomato

Journal:Agronomy-Basel|DOI:10.3390/agronomy16040420|IF:4.1

[ 66 ]The Antitumor Agent Ansamitocin P-3 Binds to Cell Division Protein FtsZ in Actinosynnema pretiosum

Journal:Biomolecules|DOI:10.3390/biom10050699|IF:4.08

[ 67 ]Fungal Gene Mutation Analysis Elucidating Photoselective Enhancement of UV-C Disinfection Efficiency Toward Spoilage Agents on Fruit Surface

Journal:Frontiers in Microbiology|DOI:10.3389/fmicb.2018.01141|IF:4.02

[ 68 ]Reprogrammed Komagataella phaffii for enhanced secretory expression of human lactoferrin

Journal:JOURNAL OF BIOTECHNOLOGY|DOI:10.1016/j.jbiotec.2026.07.011|IF:4

[ 69 ]Highly Efficient Enzymatic Catalytic Transformation for the Synthesis of Rare Ginsenoside Compound K (CK) and Its Bioactivity Study

Journal:BIOTECHNOLOGY AND BIOENGINEERING|DOI:10.1002/bit.70277|IF:3.9

[ 70 ]Identification and characterization of the vasa gene in the diamondback moth, Plutella xylostella

Journal:INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY|DOI:10.1016/j.ibmb.2020.103371|IF:3.83

[ 71 ]A point mutant in the promoter of transglutaminase gene dramatically increased yield of microbial transglutaminase from Streptomyces mobaraensis TX1

Journal:PROCESS BIOCHEMISTRY|DOI:10.1016/j.procbio.2021.11.021|IF:3.76

[ 72 ]UvATG6 Interacts with BAX Inhibitor 1 Proteins and Plays Critical Roles in Growth, Conidiation, and Virulence in Ustilaginoidea virens

Journal:Microbiology Spectrum|DOI:10.1128/spectrum.04898-22|IF:3.7

[ 73 ]Systematic Comparison and Rational Design of Theophylline Riboswitches for Effective Gene Repression

Journal:Microbiology Spectrum|DOI:10.1128/spectrum.02752-22|IF:3.7

[ 74 ]DDX1 from Cherry valley duck mediates signaling pathways and anti-NDRV activity

Journal:VETERINARY RESEARCH|DOI:10.1186/s13567-020-00889-4|IF:3.7

[ 75 ]The impact of Paenibacillus polymyxa HY96-2 luxS on biofilm formation and control of tomato bacterial wilt

Journal:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY|DOI:10.1007/s00253-019-10162-0|IF:3.67

[ 76 ]Genome mining and homologous comparison strategy for digging exporters contributing self-resistance in natamycin-producing Streptomyces strains

Journal:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY|DOI:10.1007/s00253-019-10131-7|IF:3.67

[ 77 ]The pathway of 2,2-dichlorovinyl dimethyl phosphate (DDVP) degradation by Trichoderma atroviride strain T23 and characterization of a paraoxonase-like enzyme

Journal:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY|DOI:10.1007/s00253-019-10136-2|IF:3.67

[ 78 ]Two amino acids missing of MtrA resulted in increased erythromycin level and altered phenotypes in Saccharopolyspora erythraea

Journal:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY|DOI:10.1007/s00253-019-09825-9|IF:3.67

[ 79 ]Improved PKS Gene Expression With Strong Endogenous Promoter Resulted in Geldanamycin Yield Increase

Journal:Biotechnology Journal|DOI:10.1002/biot.201700321|IF:3.65

[ 80 ]Functional characterization of bat IRF1 in IFN induction

Journal:DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY|DOI:10.1016/j.dci.2022.104500|IF:3.61

[ 81 ]A method for functional testing constitutive and ligand-induced interactions of lysin motif receptor proteins

Journal:Plant Methods|DOI:10.1186/s13007-020-0551-4|IF:3.61

[ 82 ]Enhanced Monensin Biosynthesis in Streptomyces cinnamonensis with Multigene Integration and Overexpression of Newly Identified Genes

Journal:BIOCHEMICAL ENGINEERING JOURNAL|DOI:10.1016/j.bej.2026.110118|IF:3.6

[ 83 ]Catalytic synthesis of 2,5-bis(hydroxymethyl)furan from 5-hydroxymethylfurfual by recombinant Saccharomyces cerevisiae

Journal:ENZYME AND MICROBIAL TECHNOLOGY|DOI:10.1016/j.enzmictec.2019.109491|IF:3.55

[ 84 ]Protective Role of Rabbit Nucleotide-Binding Oligomerization Domain-2 (NOD2)-Mediated Signaling Pathway in Resistance to Enterohemorrhagic Escherichia coli Infection

Journal:Frontiers in Cellular and Infection Microbiology|DOI:10.3389/fcimb.2018.00220|IF:3.52

[ 85 ]Functional characterization of a GH62 family α-L-arabinofuranosidase from Eupenicillium parvum suitable for monosaccharification of corncob arabinoxylan in combination with key enzymes

Journal:ENZYME AND MICROBIAL TECHNOLOGY|DOI:10.1016/j.enzmictec.2021.109965|IF:3.49

[ 86 ]DRAM1 deficiency affects the organization and function of the Golgi apparatus

Journal:CELLULAR SIGNALLING|DOI:10.1016/j.cellsig.2019.109375|IF:3.39

[ 87 ]High-mobility group box 1 protein (HMGB1) from Cherry Valley duck mediates signaling pathways and antiviral activity

Journal:VETERINARY RESEARCH|DOI:10.1186/s13567-020-00742-8|IF:3.36

[ 88 ]Identification of the UBA2-WTIP fusion gene in acute myeloid leukemia

Journal:EXPERIMENTAL CELL RESEARCH|DOI:10.1016/j.yexcr.2018.08.035|IF:3.31

[ 89 ]A janus kinase from Scylla paramamosain activates JAK/STAT signaling pathway to restrain mud crab reovirus

Journal:FISH & SHELLFISH IMMUNOLOGY|DOI:10.1016/j.fsi.2019.03.056|IF:3.3

[ 90 ]The Establishment of Infectious Clone and Single Round Infectious Particles for Coxsackievirus A10

Journal:VIROLOGICA SINICA|DOI:10.1007/s12250-020-00198-2|IF:3.24

[ 91 ]Identification of a Conserved Linear Antigenic Determinant in the Senecavirus A VP1 Protein

Journal:Animals|DOI:10.3390/ani16121856|IF:3.2

[ 92 ]Functional characterization of NLRX1 in rabbit during enterohemorrhagic Escherichia coli infection

Journal:DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY|DOI:10.1016/j.dci.2020.103612|IF:3.19

[ 93 ]Enhanced Efficiency of flySAM by Optimization of sgRNA Parameters in Drosophila

Journal:G3-Genes Genomes Genetics|DOI:10.1534/g3.120.401614|IF:2.78

[ 94 ]Improving sulforaphane content in transgenic broccoli plants by overexpressing MAM1, FMOGS–OX2, and Myrosinase

Journal:PLANT CELL TISSUE AND ORGAN CULTURE|DOI:10.1007/s11240-021-02079-2|IF:2.71

[ 95 ]Expression of Cytochrome P450 (CYP96A146) from flixweed (Descurainia sophia) in Saccharomyces cerevisiae and metabolism of model substrates and herbicides

Journal:WEED SCIENCE|DOI:10.1017/wsc.2026.10130|IF:2.4

[ 96 ]Organ-Wide qPCR Profiling Reveals High Trypanosome Intensity in Oral Mucosa of Cultured Larimichthys crocea

Journal:JOURNAL OF FISH DISEASES|DOI:10.1111/jfd.70248|IF:2.3