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AXL | UFO Rabbit pAb(bs-5180R)

-50ul/100ul/200ul

价格￥1180 - 2800

品牌Bioss
货号bs-5180R
更新日期2026年10月13日

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详细信息
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技术资料

规格：
50ul/100ul/200ul

规格：	50ul	产品价格：	￥1180.0
规格：	100ul	产品价格：	￥1980.0
规格：	200ul	产品价格：	￥2800.0

产品编号	bs-5180R
英文名称	AXL Rabbit pAb
中文名称	粘附相关激酶抗体
英文别名	Adhesion related kinase; AI323647; Ark; Axl; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming gene; AXL transforming sequence/gene; EC 2.7.10.1; JTK11; Oncogene AXL; Tyro7; Tyrosine protein kinase receptor UFO; Tyrosine-protein kinase receptor UFO; UFO; UFO_HUMAN.
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
交叉反应	Human, Mouse (Rat, Dog, Horse, Rabbit)
抗体来源	Rabbit
免疫原	KLH conjugated synthetic peptide derived from human AXL
亚型	IgG
性状	Liquid
纯化方法	affinity purified by Protein A
克隆类型	Polyclonal
理论分子量	95 kDa
浓度	1mg/ml
储存液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究领域	Cancer > Cell cycle > Kinases/phosphatases Cancer > Oncoproteins/suppressors > Oncoproteins > Signal transducers Cancer > Signal transduction Cell Biology > Cell Cycle > Kinases/Phosphatases Signal Transduction > Protein Phosphorylation > Tyrosine Kinases > Receptor Tyrosine Kinases Tags & Cell Markers > Cell Type Markers > Tumor Associated
亚基	Heterodimer and heterotetramer with ligand GAS6. Interacts with CBL, GRB2, LCK, NCK2, PIK3R1, PIK3R2, PIK3R3, PLCG1, SOCS1 and TENC1. Part a complex including AXL, TNK2 and GRB2, in which GRB2 promotes AXL recruitment by TNK2.
亚细胞定位	Cell membrane; Single-pass type I membrane protein.
组织特异性	Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
翻译后修饰	Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity.
相似性	Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily. _x000D_ Contains 2 fibronectin type-III domains. _x000D_ Contains 2 Ig-like C2-type (immunoglobulin-like) domains. _x000D_ Contains 1 protein kinase domain
功能	Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TENC1. Recruitment of GRB2 and phosphatidylinositol-3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. In case of filovirus infection, seems to function as a cell entry factor.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
背景资料	The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors such as vitamin K dependent protein growth arrest specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia associated oncogene and also associated with colon cancer and melanoma.The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants (AXL1 and AXL2).

应用	推荐稀释比例
{WB}	{1:500-2000}
{IHC-P}	{1:100-500}
{IHC-F}	{1:100-500}
{IF}	{1:100-500}

Tissue/cell: human colon carcinoma; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-AXL/UFO Polyclonal Antibody, Unconjugated(bs-5180R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_

Tissue/cell: human lung carcinoma; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-AXL/UFO Polyclonal Antibody, Unconjugated(bs-5180R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_

Sample:
_x000D_ MKN45(Human) Cell Lysate at 30 ug
_x000D_ SW480(Human) Cell Lysate at 30 ug
_x000D_ FHC(Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-AXL (bs-5180R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 140'120'98 kD
_x000D_ Observed band size: 120 kD
_x000D_

Sample:
_x000D_ Testis (Mouse) Lysate at 40 ug
_x000D_ Hela (Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-AXL (bs-5180R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 140/120/65 kD
_x000D_ Observed band size: 120 kD

Sample:
_x000D_ Lovo(Human) Cell Lysate at 40 ug
_x000D_ K562(Human) Cell Lysate at 40 ug
_x000D_ Primary: Anti-AXL (bs-5180R) at 1/500 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 95 kD
_x000D_ Observed band size: 95 kD

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