Blank control:A549.
Primary Antibody (green line): Rabbit Anti-PTGES2 antibody (bs-2639R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
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- bs-2639R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2639R |
| 英文名称 | PTGES2 Rabbit pAb |
| 中文名称 | 前列腺素E合成酶2抗体 |
| 英文别名 | C9orf15; FLJ14038; gamma interferon activated transcriptional element binding factor 1; GATE binding factor 1; GBF 1; GBF1; Membrane associated prostaglandin E synthase 2; MGC11289; Microsomal prostaglandin E synthase 2; mPGES 2; mPGES-2; PGES2; PGES2_HUMAN; Prostaglandin E synthase 2; Prostaglandin E synthase 2 truncated form; PTGES 2; PGES2_MOUSE. |
| 产品应用 | Flow-Cyt=1ug/test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Chicken, Dog, Pig, Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from mouse PTGES2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 43 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Homodimer. May interact with CEBPB. |
| 亚细胞定位 | Golgi apparatus membrane; Single-pass membrane protein. Nucleus. Note=According to PubMed:12050152, some fraction may be nuclear. Prostaglandin E synthase 2 truncated form: Cytoplasm. Note=Synthetized as a Golgi membrane-bound protein, which is further cleaved into the predominant soluble truncated form. |
| 组织特异性 | Widely expressed. Expressed in brain, heart, liver, colon and lung. |
| 相似性 | Belongs to the GST superfamily.
Contains 1 glutaredoxin domain. Contains 1 GST C-terminal domain. |
| 功能 | Isomerase that catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form (By similarity). May also have transactivation activity toward IFN-gamma (IFNG), possibly via an interaction with CEBPB; however, the relevance of transcription activation activity remains unclear. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a membrane-associated prostaglandin E synthase, which catalyzes the conversion of prostaglandin H2 to prostaglandin E2. This protein also has been shown to activate the transcription regulated by a gamma-interferon-activated transcription element (GATE). Multiple transcript variants have been found for this gene. [provided by RefSeq]. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1ug/test} |
Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-PTGES2 antibody (bs-2639R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-PTGES2 antibody (bs-2639R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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文献和实验该产品被引用文献
[IF={{ 7.31 }}] {Yu Liu. et al. Inhibition of Prostaglandin E2 Receptor EP3 Attenuates Oxidative Stress and Neuronal Apoptosis Partially by Modulating p38MAPK/FOXO3/Mul1/Mfn2 Pathway after Subarachnoid Hemorrhage in Rats. OXID MED CELL LONGEV. 2022 Dec 09;2022:7727616} {WB} {Rat}
[IF={{ 5.9 }}] {Soon-Young Lee. et al. Ginseng Berry Juice (GBJ) Regulates the Inflammation in Acute Ulcerative Mouse Models and the Major Bioactive Substances Are Ginsenosides Rb3, Rc, Rd, and Re. NUTRIENTS. 2024 Jan;16(7):1031} {IHC} {Mouse}
[IF={{ 5.7 }}] {Lee Soon-Young. et al. Oleic acid attenuates asthma pathogenesis via Th1/Th2 immune cell modulation, TLR3/4-NF-κB-related inflammation suppression, and intrinsic apoptotic pathway induction. FRONT IMMUNOL. 2024 Oct;15:} {WB,IHC} {Mouse}
[IF={{ 5.6 }}] {Soon-Young Lee. et al. Saururus chinensis water-extract effectively controls asthma by ameliorating of Th1/Th2 imbalance and suppressing of NF-κB/COX-2/PGE2-related inflammation. J FUNCT FOODS. 2024 Jan;112:105930} {IHC} {Mouse}
[IF={{ 5.487 }}] {Soon-Young Lee. et al. Korean Red Ginseng affects ovalbumin-induced asthma by modulating IL-12, IL-4, and IL-6 levels and the NF-κB/COX-2 and PGE2 pathways. J Ginseng Res. 2020 Oct;:} {IHC} {Mouse}
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PTGES2 Rabbit pAb(bs-2639R)-50ul/100ul/200ul
￥1180 - 2800
