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TNFSF11 | RANKL/CD254 Rabbit p

Ab(bs-0747R)-50ul/100ul/200ul

价格￥1180 - 2800

品牌Bioss
货号bs-0747R
更新日期2026年10月13日

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规格：
50ul/100ul/200ul

规格：	50ul	产品价格：	￥1180.0
规格：	100ul	产品价格：	￥1980.0
规格：	200ul	产品价格：	￥2800.0

产品编号	bs-0747R
英文名称	RANKL/CD254 Rabbit pAb
中文名称	骨保护蛋白配体/破骨细胞分化因子抗体
英文别名	OPGL; CD254; hRANKL2; ODF; OPGL; Osteoclast differentiation factor; Osteoprotegerin ligand; RANKL; Receptor activator of nuclear factor kappa B ligand; sOdf; SOFA; TNF related activation induced cytokine; TNFSF 11; TNFSF11; TRANCE; Tumor necrosis factor ligand superfamily member 11; Osteoprotegerin Ligand; TNF11_HUMAN.
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
交叉反应	Human, Mouse (Rat, Dog)
抗体来源	Rabbit
免疫原	KLH conjugated synthetic peptide derived from human OPGL
亚型	IgG
性状	Liquid
纯化方法	affinity purified by Protein A
克隆类型	Polyclonal
理论分子量	35 kDa
浓度	1mg/ml
储存液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究领域	Cardiovascular > Atherosclerosis > Thrombosis Immunology > Adaptive Immunity > Regulatory T Cells Immunology > Innate Immunity > Cytokines > TNF Superfamily
亚细胞定位	Cytoplasm; Secreted and Cell membrane.
组织特异性	Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.
翻译后修饰	The soluble form of isoform 1 derives from the membrane form by proteolytic processing. The cleavage may be catalyzed by ADAM17.
相似性	Belongs to the tumor necrosis factor family.
功能	Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
背景资料	This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq, Jul 2008].

应用	推荐稀释比例
{WB}	{1:500-2000}
{IHC-P}	{1:100-500}
{IHC-F}	{1:100-500}
{IF}	{1:100-500}
{Flow-Cyt}	{1μg/Test }

Tissue/cell: human gastric carcinoma; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-OPGL/RANKL/ODF Polyclonal Antibody, Unconjugated(bs-0747R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_

Tissue/cell: mouse spleen tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-OPGL/RANKL/ODF Polyclonal Antibody, Unconjugated(bs-0747R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_

Blank control: mouse splenocytes(blue)
_x000D_ Isotype Control Antibody: Rabbit IgG(orange) ; _x000D_ Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), _x000D_ Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; _x000D_ Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green)._x000D_

Sample:
_x000D_ HUVEC-C Cell (Human) Lysate at 40 ug
_x000D_ HepG2 Cell (Human) Lysate at 40 ug
_x000D_ Primary: Anti-RANKL/CD254 (bs-0747R)at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 35kD
_x000D_ Observed band size: 56kD
_x000D_

Sample:
_x000D_ Lymph node(Mouse) Lysate at 40 ug
_x000D_ Thymus(Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-RANKL'CD254 (bs-0747R) at 1/500 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 37'50 kD
_x000D_ Observed band size: 50 kD
_x000D_

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