小鼠白介素6试剂盒Mouse IL-6 ELISA Kit

背景介绍	白细胞介素6(IL-6)是一种同时具备促炎性和抗炎性的细胞因子。在人类中，它由IL-6基因编码。在感染期间和受到外伤后，尤其是烧伤或其它组织损伤导致的炎症，IL-6会由T细胞和巨噬细胞分泌，促进免疫应答。研究表明，小鼠抵抗肺炎链球菌感染时需要IL-6的参与，说明IL-6在抵抗感染中发挥作用。   IL-6在很多疾病如糖尿病、动脉粥样硬化、抑郁症、阿尔茨海默氏病、系统性红斑狼疮、多发性骨髓瘤、前列腺癌、白塞氏病和类风湿性关节炎等中可促进炎性和自身免疫疾病进程。晚期/转移性癌症患者的血液中具有较高水平的IL-6。
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-6抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-6与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-6的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
组分	包被抗IL-6单克隆抗体的96孔聚苯乙烯酶标板 小鼠IL-6冻干标准品 IL-6检测抗体 标准品稀释液 检测缓冲液(10×) 底物(TMB) 终止液 洗液(20×) 封板膜
分析类型	夹心ELISA
样本类型	血清、血浆，细胞培养上清及其他生物学样本
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
样本体积	10 μl
灵敏度	0.56 pg/ml
标曲图
标准曲线范围	7.81 - 500 pg/ml
回收率范围	89 %-112 %
平均回收率	1.02
板间变异系数	2.3 % - 3 %
板内变异系数	2.1 % - 5.3 %

小鼠白介素6 ELISA试剂盒说明书

本产品文献引用13篇，代表文献如下：

【1】Jun-Xia Zheng, Yu-Shui Han, Jin-Cai Wang, et al. Strigolactones: a plant phytohormone as novel anti-inflammatory agents[J]. MedChemCommun, 2018, 9: 181-188 (IF:5.197)

【2】BaidaKong, XiaWang, Bingnan, et al. 8:2 fluorotelomer alcohol inhibited proliferation and disturbed the expression of pro-inflammatory cytokines and antigen-presenting genes in murine macrophages[J]. Chemosphere, 2019, 219: 1052-1060 (IF:4.427)

【3】Lingman Maa, Lifen Qianb, Qidi Yingb, et al. I 4,a synthetic anti-diabetes agent, attenuates atherosclerosis through its lipid-lowering, anti-inflammatory and anti-apoptosis properties[J]. Molecular and Cellular Endocrinology, 2017, 440: 80-92 (IF:3.859)

【4】Deng J, Zeng L, Lai X, et al. Metformin protects against intestinal barrier dysfunction\r, via\r, AMPKα1-dependent inhibition of JNK signalling activation[J]. Journal of Cellular and Molecular Medicine, 2018, 22(1): 546-557 (IF:5.95)

【5】Li X, Ying-Fan H, Jia-Si W, et al. Semi-Mechanism-Based Pharmacodynamic Model for the Anti-Inflammatory Effect of Baicalein in LPS-Stimulated RAW264.7 Macrophages[J]. Frontiers in Pharmacology, 2018, 9: 793 (IF:3.831)

IL6 分子靶点信息概述

• 分子名：IL6, interleukin 6
• 基因家族：Interferons; Interleukins; Interleukin 6 type cytokine family
• 别名：IL-6; BSF2; HGF; HSF
• 曾用名：IFNB2
• 全称：interferon, beta 2; interleukin 6 (interferon, beta 2)

IL6 分子靶点综述

白细胞介素6(IL-6)是一种同时具备促炎性和抗炎性的细胞因子。在人类中，它由IL-6基因编码。在感染期间和受到外伤后，尤其是烧伤或其它组织损伤导致的炎症，IL-6会由T细胞和巨噬细胞分泌，促进免疫应答。研究表明，小鼠抵抗肺炎链球菌感染时需要IL-6的参与，说明IL-6在抵抗感染中发挥作用。IL-6在很多疾病如糖尿病、动脉粥样硬化、抑郁症、阿尔茨海默氏病、系统性红斑狼疮、多发性骨髓瘤、前列腺癌、白塞氏病和类风湿性关节炎等中可促进炎性和自身免疫疾病进程。晚期/转移性癌症患者的血液中具有较高水平的IL-6。

小鼠 Mouse Il6 分子靶点信息

• 分子名：Il6, interleukin 6
• 别称：
  • Il-6
• 基因序列：NCBI_Gene: 16193
• 蛋白序列：
  • UniprotKB: P08505
  • UniprotKB: A0A0G2JFG1
  • UniprotKB: A0A0G2JFT1
  • UniprotKB: A0A0G2JGF4
  • UniprotKB: A2RTD1
  • UniprotKB: Q0PMN1
  • UniprotKB: Q3U8J8

小鼠 Mouse Il6 靶点分子功能（预测）

Enables cytokine activity and interleukin-6 receptor binding activity. Involved in several processes, including animal organ development; positive regulation of macromolecule metabolic process; and regulation of peptide hormone secretion. Acts upstream of or within with a positive effect on gene expression. Acts upstream of or within several processes, including cellular response to cytokine stimulus; regulation of gene expression; and salivary gland morphogenesis. Located in external side of plasma membrane and extracellular space. Part of interleukin-6 receptor complex. Is expressed in several structures, including adrenal gland; genitourinary system; gut; liver; and thymus. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Orthologous to human IL6 (interleukin 6).