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- MultiSciences
- 0.31 ng/mL - 20 ng/mL
- 杭州
- EK2173
- 2026年03月23日
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- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
联科生物
- 检测范围：
0.31 ng/mL - 20 ng/mL
- 检测方法：
双抗夹心法
- 应用：
ELISA
- 适应物种：
Mouse
- 标记物：
HRP
- 样本：
血清，血浆，细胞培养上清及其他生物学样本
- 规格：
96T/48T
| 规格： | 96T | 产品价格： | ￥2650.0 |
|---|---|---|---|
| 规格： | 48T | 产品价格： | ￥1600.0 |
| 背景介绍 | 颗粒酶B(GranzymeB)是一种丝氨酸蛋白酶，最常见于细胞毒性淋巴细胞(CTLs)和自然杀伤细胞(NK细胞)颗粒中。由这些细胞分泌的GranzymeB和穿孔蛋白一起作用介导靶细胞的凋亡。最近，人们发现许多非细胞毒性细胞从嗜碱性粒细胞、肥大细胞到平滑肌细胞也可产生GranzymeB。GranzymeB通过刺激细胞因子的释放参与诱导炎症，同样也参与细胞外基质重塑。升高的GranzymeB水平与许多自身免疫性疾病、一些皮肤病和1型糖尿病有关。 |
| 检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附法定量检测小鼠Granzyme B。小鼠Granzyme B试剂盒仅供科学研究，不用于临床诊断。 颗粒酶 B (Granzyme B)是一种丝氨酸蛋白酶，最常见于细胞毒性淋巴细胞(CTLs)和自然杀伤 细胞(NK 细胞)颗粒中。 由这些细胞分泌的 Granzyme B 和穿孔蛋白一起作用介导靶细胞的凋亡。 最近，人们发现许多非细胞毒性细胞从嗜碱性粒细胞、肥大细胞到平滑肌细胞也可产生 Granzyme B。 Granzyme B 通过刺激细胞因子的释放参与诱导炎症， 同样也参与细胞外基质重塑。 升高的 Granzyme B 水平与许多自身免疫性疾病、 一些皮肤病和 1 型糖尿病有关。 |
| 组分 | 包被抗Granzyme B单克隆抗体的96孔聚苯乙烯酶标板 小鼠Granzyme B冻干标准品 Granzyme B检测抗体 辣根过氧化物酶标记的链霉亲和素 检测缓冲液(10×) 底物(TMB) 终止液 洗液(20×) 封板膜 |
| 分析类型 | 夹心ELISA |
| 样本类型 | 血清、血浆，细胞培养上清及其他生物学样本 |
| 板式 | 96孔板，可拆 |
| 保存 | 试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。 |
| 运输条件 | 4℃蓝冰运输 |
| 样本体积 | 10 μl |
| 灵敏度 | 0.91 pg/ml |
| 标曲图 |  |
| 标准曲线范围 | 0.31 - 20ng/ml |
| 回收率范围 | 91 %-105 % |
| 平均回收率 | 0.99 |
| 板间变异系数 | 1.8 % - 2.8 % |
| 板内变异系数 | 2.9 % - 3.9 % |
GZMB 分子靶点信息概述
- 分子名：GZMB, granzyme B
- 基因家族：Granule associated serine proteases of immune defence
- 别名：CCPI; CGL-1; CSP-B; CGL1; CTSGL1; HLP; SECT
- 曾用名：CTLA1; CSPB
- 全称：fragmentin 2; cytotoxic serine protease B; cathepsin G-like 1; T-cell serine protease 1-3E; granzyme 2; cytotoxic T-lymphocyte-associated serine esterase 1; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
GZMB 分子靶点综述
颗粒酶B(GranzymeB)是一种丝氨酸蛋白酶，最常见于细胞毒性淋巴细胞(CTLs)和自然杀伤细胞(NK细胞)颗粒中。由这些细胞分泌的GranzymeB和穿孔蛋白一起作用介导靶细胞的凋亡。最近，人们发现许多非细胞毒性细胞从嗜碱性粒细胞、肥大细胞到平滑肌细胞也可产生GranzymeB。GranzymeB通过刺激细胞因子的释放参与诱导炎症，同样也参与细胞外基质重塑。升高的GranzymeB水平与许多自身免疫性疾病、一些皮肤病和1型糖尿病有关。
小鼠 Mouse Gzme 分子靶点信息
- 分子名：Gzme, granzyme E
- 别称：
- CCP3
- Ctla-6
- Ctla6
- cytotoxic T lymphocyte-associated protein 6
- MCSP-2
- 基因序列：NCBI_Gene: 14942
- 蛋白序列：
- UniprotKB: P08884
小鼠 Mouse Gzme 靶点分子功能（预测）
Predicted to enable serine-type endopeptidase activity. Predicted to be involved in several processes, including granzyme-mediated programmed cell death signaling pathway; natural killer cell mediated cytotoxicity; and pyroptosis. Predicted to act upstream of or within cytolysis. Predicted to be located in cytolytic granule. Predicted to be active in cytoplasm and intracellular membrane-bounded organelle. Is expressed in extraembryonic component; placenta; spongiotrophoblast; and trophoblast giant cell. Orthologous to human GZMB (granzyme B).
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