- 询价
- Cell Signaling Technology
- 9644
- USA
- 2025年08月18日
- W, IP, IHC-P, IF-IC, F
- H,M,R,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
4E-BP1 (53H11) Rabbit mAb
- 抗原：
synthetic peptide corresponding to residues surrounding Ser112 of human 4E-BP1
- 应用范围：
W, IP, IHC-P, IF-IC, F
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 适应物种：
H,M,R,Mk
- 库存：
大量
- 供应商：
CST
- 是否单克隆：
0
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | H M R Mk | Endogenous | 15-20 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | 4E-BP1 (53H11) Rabbit mAb detects endogenous levels of total 4E-BP1 protein. |
| Source / Purification | 4E-BP1 (53H11) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding Ser112 of human 4E-BP1. Western Blotting
Western blot analysis of extracts from various cell lines using 4E-BP1 (53H11) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using 4E-BP1 (53H11) Rabbit mAb in the presence of control peptide (left) or 4E-BP1 blocking peptide #1053 (right). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using 4E-BP1 (53H11) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using 4E-BP1 (53H11) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse spleen, 4E-BP1/2 wild type (left) or 4E-BP1 knockout (right), using 4E-BP1 (53H11) Rabbit mAb. 4E-BP wild type and knockout tissues kindly provided by Dr. Nahum Sonenberg, McGill University. Flow Cytometry
Flow cytometric analysis of COS cells using 4E-BP1 (53H11) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). IF-IC
Confocal immunofluorescent analysis of HeLa cells using 4E-BP1 (53H11) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). |
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063. For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Generation of Rabbit Immune Libraries
Rabbits provide an interesting alternative to antibodies derived from the murine system. Furthermore, rabbits show an exceptional feature with respect to the mechanisms that are used for the generation of antibody diversity, i.e. a single VH
T-Cell Activation Using mAb to CD3
µg/ml solution of anti-CD3e (145-2C11) in sterile PBS. Calculate the number of wells required for each experimental condition and consider triplicate samples for each condition. For example, to coat one-half plate (48 wells) 2.6ml of antibody solution
In Vitro Translation Using Rabbit Reticulocyte Lysate
. This eliminates any background translation products. Several factors are added to rabbit reticulocyte lysate to optimize translation efficiency. These include hemin (to prevent phosphorylation of initiation factor eIF2-a), an energy-generating system (e.g
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