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TFIIF-α Antibody

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  • 询价
  • Cell Signaling Technology已认证
  • USA
  • 2025年10月15日
  • W, IP
  • Rabbit
  • H,Mk,Dg
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体英文名

      TFIIF-α Antibody

    • 抗原

      synthetic peptide corresponding to residues near the amino terminus of human TFIIF-α protein

    • 应用范围

      W, IP

    • 宿主

      Rabbit

    • 库存

      大量

    • 适应物种

      H,Mk,Dg

    • 级别

      详见MSDS文件

    • 保质期

      详见说明书

    • 供应商

      CST

    • 是否单克隆

      2

    • 保存条件

      -20°c

    • 规格

      100 ul (10 western blots)/carrier free & custom formulation / quantity

    规格:产品价格:¥请询价
    规格:100 ul (10 western blots)产品价格:¥请询价
    规格:carrier free & custom formulation / quantity产品价格:¥请询价

    pathway more info application references datasheet PDF MSDS PDF protocols

    Applications Key:  W=Western Blotting  IP=Immunoprecipitation
    Reactivity Key:  H=Human  Mk=Monkey  Dg=Dog
    Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

    Applications Reactivity Sensitivity MW (kDa) Source
    W IP H Mk (Dg) Endogenous 74 Rabbit
    Protocols
    Specificity / Sensitivity

    TFIIF-α Antibody recognizes endogenous levels of total TFIIF-α protein.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TFIIF-α protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Western Blotting

    Western Blotting

    Western blot analysis of extracts from various cell lines using TFIIF-α Antibody.

    Background

    TFIIF is a member of the group of general transcription factors that facilitate the binding of RNA polymerase II (Pol II) to promoter sequences as part of the pre-initiation complex (PIC) (1). TFIIF consists of subunits TFIIF-α (RAP74) and TFIIF-β (RAP30). It is involved in the stabilization of Pol II association with the PIC and selection of the transcription start site during transcription initiation (1,2). In addition to its role in transcription initiation, TFIIF has been shown to stimulate the transcription elongation activity of Pol II as well as dephosphorylation and recycling of Pol II during transcription termination (3-5).

    1. Thomas, M.C. and Chiang, C.M. Crit Rev Biochem Mol Biol 41, 105-78.
    2. Hahn, S. (2004) Nat Struct Mol Biol 11, 394-403.
    3. Sims, R.J. et al. (2004) Genes Dev 18, 2437-68.
    4. Zhang, C. and Burton, Z.F. (2004) J Mol Biol 342, 1085-99.
    5. Zawel, L. et al. (1995) Genes Dev 9, 1479-90.
    Application References

    Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

    Companion Products

    For Research Use Only. Not For Use In Diagnostic Procedures.

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