Snail (C15D3) Rabbit mAb产品图

Snail (C15D3) Rabbit mAb

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  • 询价
  • Cell Signaling Technology已认证
  • USA
  • 2026年05月29日
  • W, IP
  • H,M,R,Mk
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    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 抗体英文名

      Snail (C15D3) Rabbit mAb

    • 抗原

      recombinant human Snail protein

    • 应用范围

      W, IP

    • 级别

      详见MSDS文件

    • 供应商

      CST

    • 保质期

      详见说明书

    • 适应物种

      H,M,R,Mk

    • 库存

      大量

    • 是否单克隆

      1

    • 保存条件

      -20°c

    • 规格

      40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity

    规格:产品价格:¥请询价
    规格:40 ul (4 western blots)产品价格:¥请询价
    规格:100 ul (10 western blots)产品价格:¥请询价
    规格:carrier free & custom formulation / quantity产品价格:¥请询价

    pathway more info application references datasheet PDF MSDS PDF protocols

    Applications Key:  W=Western Blotting  IP=Immunoprecipitation
    Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
    Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

    Applications Reactivity Sensitivity MW (kDa) Isotype
    W IP H M R Mk Endogenous 29 Rabbit IgG
    Protocols
    Specificity / Sensitivity

    Snail (C15D3) Rabbit mAb detects endogenous levels of total Snail protein.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant human Snail protein.

    Western Blotting

    Western Blotting

    Western blot analysis of extracts from various cell lines using Snail (C15D3) Rabbit mAb.

    Background

    Snail is a zinc-finger transcription factor that can repress E-cadherin transcription. Downregulation of E-cadherin is associated with epithelial-mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells (1-3). Indeed, loss of E-cadherin expression is correlated with the invasive properties of some tumors and there is a considerable inverse correlation between Snail and E-cadherin mRNA levels in epithelial tumor cell lines (4,5). In addition, Snail blocks the cell cycle and confers resistance to cell death (6). Phosphorylation of Snail by GSK-3 and PAK1 regulates its stability, cellular localization and function (7-10).

    1. Barrallo-Gimeno, A. and Nieto, M.A. (2005) Development 132, 3151-3161.
    2. Cano, A. et al. (2000) Nat. Cell Biol. 2, 76-83.
    3. Batlle, E. et al. (2000) Nat. Cell Biol. 2, 84-89.
    4. Yokoyama, K. et al. (2003) Int. J. Oncol. 22, 891-898.
    5. Yokoyama, K. et al. (2001) Oral Oncol. 37, 65-71.
    6. Vega, S. et al. (2004) Genes Dev. 18, 1131-1143.
    7. Zhou, B.P. et al. (2004) Nat. Cell Biol. 6, 931-940.
    8. Bachelder, R.E. et al. (2005) J. Cell Biol. 168, 29-33.
    9. Yook, J.I. et al. (2005) J. Biol. Chem. 280, 11740-11748.
    10. Yang, Z. et al. (2005) Cancer Res. 65, 3179-3184.
    Application References

    Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

    Companion Products

    For Research Use Only. Not For Use In Diagnostic Procedures.

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    • 作者
    • 内容
    • 询问日期
    图标文献和实验
    该产品被引用文献
    [1]Developing a prognostic stratification model based on glutathione metabolism in thyroid cancer and validating RRM2's tumor-promoting role.
    PMID:41333212Front Oncol2025-11-17
    [2]A CEBPB/TYMP/GDF15 signaling axis mediates tumor growth and cisplatin resistance in bladder cancer.
    PMID:40957398Transl Oncol2025-11
    [3]CYMP-AS1 Promotes Ovarian Cancer Progression by Enhancing the Intracellular Translocation of hnRNPM and Reducing the Stability of AXIN2 mRNA.
    PMID:40746892Oncol Res2025-07-18
    [4]CD151 Promotes Cancer Progression in Triple-Negative Breast Cancer by Inducing EMT through the MAPK Signaling Pathway.
    PMID:40458675Breast Cancer (Dove Med Press)2025-05-28
    [5]RAB26 promotes prostate cancer progression via the MAPK/ERK-TWIST1 signaling axis.
    PMID:40821113Genes Dis2025-05-22
    [6]Hsa_circ_0051908 Promotes Hepatocellular Carcinoma Progression by Regulating the Epithelial-Mesenchymal Transition Process.
    PMID:38715919Anal Cell Pathol (Amst)2024-04-30
    [7]The Antimicrobial Peptide Merecidin Inhibit the Metastasis of Triple-Negative Breast Cancer by Obstructing EMT via miR-30d-5p/Vimentin.
    PMID:39267432Technol Cancer Res Treat2024-01-23
    [8]Aspartate β-hydroxylase Regulates Expression of Ly6 Genes.
    PMID:38356711J Cancer2024-01-01
    [9]Ophiopogonin B inhibits migration and invasion in non-small cell lung cancer cells through enhancing the interaction between Axin and β-catenin.
    PMID:34539900J Cancer2021-08-28
    [10]CCNG1 (Cyclin G1) regulation by mutant-P53 via induction of Notch3 expression promotes high-grade serous ovarian cancer (HGSOC) tumorigenesis and progression.
    PMID:30565428Cancer Med2019-01
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    • 三生三世,一场组化,一次豪赌

      h)即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复:简单易行效果好,CST 推荐使用微波炉完成修复。合适的修复液:根据抗体说明书使用合适的修复液。用柠檬酸修复后,切片需浸泡在修复液中,自然冷却;而用 EDTA 修复后,切片可直接从修复缸中取出,直接进行下一步。注:使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb(#4267),EDTA 的修复方式明显优于柠檬酸盐及胃蛋白

    • 手把手课程之 IHC 关键操作步骤

      。所以,我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体:Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本:石蜡包埋人类肺部肿瘤样本煮沸设备:浴锅 (左),微波炉 (中),高压锅 (右)免疫染色—封闭在 IHC–P 中,我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品,时长 1 小时,以防止非特异性背景染色。在进行 IHC–F 时,在含有 0.3% Triton. X–100 的 1X

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