HEXIM1 Antibody

9064:

Immunoprecipitation , Western Blotting

HEXIM1 Antibody recognizes endogenous levels of total HEXIM1 protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human HEXIM1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from various cell lines using HEXIM1 Antibody.

Hexamethylene bisacetamide-induced protein 1 (HEXIM1) was originally identified in vascular smooth muscle cells as a protein that is upregulated upon treatment with the differentiating agent hexamethylene bisacetamide (1). HEXIM1 binds to 7SK RNA, a highly abundant non-coding RNA, and together act as a potent inhibitor of positive transcription elongation b (P-TEFb) (2,3). P-TEFb phosphorylates the C-terminal domain of the largest subunit of RNA polymerase II and is an important regulator of transcription elongation (4-8). 7SK RNA-bound HEXIM1 interacts with the Cyclin T1 subunit of P-TEFb, sequestering it in an inactive form leading to transcription inhibition (2,3). The regulation of the relative ratio of inactive to active P-TEFb in the cell by HEXIM1/7SK RNA is thought to play a critical role in regulation of a wide range of cellular gene expression programs such as estrogen and glucocorticoid receptor regulated genes (9-12).

1. Ouchida, R. et al. (2003) Genes Cells 8, 95-107.
2. Michels, A.A. et al. (2004) EMBO J 23, 2608-19.
3. Yik, J.H. et al. (2003) Mol Cell 12, 971-82.
4. Buratowski, S. (2009) Mol Cell 36, 541-6.
5. Lenasi, T. and Barboric, M. RNA Biol 7, 145-50.
6. Pirngruber, J. et al. (2009) Cell Cycle 8, 3636-42.
7. Wada, T. et al. (1998) EMBO J 17, 7395-403.
8. Yamada, T. et al. (2006) Mol Cell 21, 227-37.
9. Peterlin, B.M. et al. (2012) Wiley Interdiscip Rev RNA 3, 92-103.
10. Ketchart, W. et al. (2011) Oncogene 30, 3563-9.
11. Ogba, N. et al. (2008) Cancer Res 68, 7015-24.
12. Shimizu, N. et al. (2005) Proc Natl Acad Sci U S A 102, 8555-60.

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For Research Use Only. Not For Use In Diagnostic Procedures.