PRK2 Antibody

PRK2 Antibody

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  • 询价
  • Cell Signaling Technology已认证
  • USA
  • 2025年09月02日
  • W, IP
  • Rabbit
  • H,M,R
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    • 详细信息
    • 技术资料
    • 抗体英文名

      PRK2 Antibody

    • 抗原

      synthetic peptide corresponding to the amino-terminal sequence of human PRK2

    • 应用范围

      W, IP

    • 宿主

      Rabbit

    • 级别

      详见MSDS文件

    • 供应商

      CST

    • 库存

      大量

    • 适应物种

      H,M,R

    • 保质期

      详见说明书

    • 是否单克隆

      2

    • 保存条件

      -20°c

    • 规格

      100 ul (10 western blots)/carrier free & custom formulation / quantity

    规格:产品价格:¥请询价
    规格:100 ul (10 western blots)产品价格:¥请询价
    规格:carrier free & custom formulation / quantity产品价格:¥请询价

    pathway more info application references datasheet PDF MSDS PDF protocols

    Applications Key:  W=Western Blotting  IP=Immunoprecipitation
    Reactivity Key:  H=Human  M=Mouse  R=Rat
    Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

    Applications Reactivity Sensitivity MW (kDa) Source
    W IP H M R Endogenous 140 Rabbit
    Protocols
    Specificity / Sensitivity

    PRK2 Antibody detects endogenous levels of total PRK2 protein. The antibody does not cross-react with PRK1.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human PRK2. Antibodies are purified by protein A and peptide affinity chromatography.

    Western Blotting

    Western Blotting

    Western blot analysis of extracts from various cell lines, using PRK2 Antibody.

    Background

    The protein kinase C-related kinases (PRKs) are a subfamily of Ser/Thr-specific kinases with a catalytic domain highly homologous to the PKC family (1-3). They are effectors of Rho family GTPases (4-6) and are activated by fatty acids and phospholipids in vitro (7,8). Activation in vitro and in vivo involves the activation loop phosphorylation of PRK1 (Thr774)/PRK2 (Thr816) by PDK1 (9,10).

    1. Mukai, H. et al. (1994) Biochem. Biophys. Res. Commun. 199, 897-904.
    2. Morrice, N.A. et al. (1994) J. Biol. Chem. 269, 20040-20046.
    3. Palmer, R.H. et al. (1994) FEBS Lett. 356, 5-8.
    4. Watanabe, G. et al. (1996) Science 271, 645-648.
    5. Amano, M. et al. (1996) Science 271, 648-650.
    6. Vincent, S. and Settleman, J. (1997) Mol. Cell. Biol. 17, 2247-2256.
    7. Morrice, N.A. et al. (1994) FEBS Lett. 351, 171-175.
    8. Palmer, R.H. et al. (1995) J. Biol. Chem. 270, 22412-22416.
    9. Flynn, P. et al. (2000) J. Biol. Chem. 275, 11064-70.
    10. Dong, L.Q. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 5089-94.
    Application References

    Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

    Companion Products

    For Research Use Only. Not For Use In Diagnostic Procedures.

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