PERK (C33E10) Rabbit mAb产品图

PERK (C33E10) Rabbit mAb

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  • 询价
  • Cell Signaling Technology已认证
  • USA
  • 2026年05月29日
  • W
  • H,M,R,Mk
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    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 抗体英文名

      PERK (C33E10) Rabbit mAb

    • 抗原

      synthetic peptide corresponding to the sequence of human PERK

    • 应用范围

      W

    • 级别

      详见MSDS文件

    • 库存

      大量

    • 保质期

      详见说明书

    • 供应商

      CST

    • 适应物种

      H,M,R,Mk

    • 是否单克隆

      1

    • 保存条件

      -20°c

    • 规格

      100 ul (10 western blots)/carrier free & custom formulation / quantity

    规格:产品价格:¥请询价
    规格:100 ul (10 western blots)产品价格:¥请询价
    规格:carrier free & custom formulation / quantity产品价格:¥请询价

    pathway more info application references datasheet PDF MSDS PDF protocols

    Applications Key:  W=Western Blotting
    Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
    Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

    Applications Reactivity Sensitivity MW (kDa) Isotype
    W H M R Mk Endogenous 140 Rabbit IgG
    Protocols
    Specificity / Sensitivity

    PERK (C33E10) Rabbit mAb detects endogenous levels of total PERK protein.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PERK.

    Western Blotting

    Western Blotting

    Western blot analysis of extracts from various cell types using PERK (C33E10) Rabbit mAb.

    Background

    PERK (protein kinase-like endoplasmic reticulum kinase) is an eIF2α kinase and transmembrane protein resident in the endoplasmic reticulum (ER) membrane that couples ER stress signals to translation inhibition (1-3). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation. PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress (4). PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain (1-3). Phosphorylation of PERK at Thr980 serves as a marker for its activation status.

    1. Harding, H. et al. (1999) Nature 397, 271-274.
    2. Shi, Y. et al. (1998) Mol. Cell. Biol. 18, 7499-7509.
    3. Harding, H. et al. (2000) Mol. Cell 5, 897-904.
    4. Harding, H. et al. (2001) Mol. Cell 7, 1153-1163.
    Application References

    Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

    Companion Products

    For Research Use Only. Not For Use In Diagnostic Procedures.

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    • 作者
    • 内容
    • 询问日期
    图标文献和实验
    该产品被引用文献
    [1]A commensal protozoan exacerbates acetaminophen-induced liver injury by producing free sphingosine
    PMID:42231521Gut Microbes2026-12-31
    [2]Neuronal SEL1L-HRD1 ER-associated degradation is essential for motor function and survival in mice
    PMID:41712288J Clin Invest2026-02-19
    [3]Lactobacillus rhamnosus GG Supernatant Improves GLP-1 Secretion Through Attenuating L Cell Lipotoxicity and Modulating Gut Microbiota in Obesity.
    PMID:40366615Probiotics Antimicrob Proteins2026-01
    [4]SREBF1 mediates immunoparalysis of dendritic cells in sepsis by regulating lipid metabolism and endoplasmic reticulum stress.
    PMID:40524162Cell Commun Signal2025-06-16
    [5]Flavin-containing monooxygenase 2 confers cardioprotection in ischemia models through its disulfide bond catalytic activity
    PMID:39480513J Clin Invest2024-10-31
    [6]Trimethylamine N-oxide impairs β-cell function and glucose tolerance.
    PMID:38514666Nat Commun2024-03-21
    [7]Gα12 and endoplasmic reticulum stress-mediated pyroptosis in a single cycle of dextran sulfate-induced mouse colitis.
    PMID:38491049Sci Rep2024-03-15
    [8]Biallelic Cys141Tyr variant of SEL1L is associated with neurodevelopmental disorders, agammaglobulinemia, and premature death
    PMID:37943617J Clin Invest2024-01-16
    [9]Inhibiting the IRE1α Axis of the Unfolded Protein Response Enhances the Antitumor Effect of AZD1775 in TP53 Mutant Ovarian Cancer.
    PMID:35619328Adv Sci (Weinh)2022-07
    [10]Hepatitis C virus inhibits AKT-tuberous sclerosis complex (TSC), the mechanistic target of rapamycin (MTOR) pathway, through endoplasmic reticulum stress to induce autophagy.
    PMID:23169238Autophagy2013-02
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