- ￥580 - 10980
- Sino Biological
- 北京
- 10602-HNAE
- 2026年01月26日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human TNF-alpha / TNFA Protein
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1.00 mg/10.00 µg/50.00 µg/100.00 µg/500.00 µg
| 规格： | 1.00 mg | 产品价格： | ￥10980.0 |
|---|---|---|---|
| 规格： | 10.00 µg | 产品价格： | ￥580.0 |
| 规格： | 50.00 µg | 产品价格： | ￥1380.0 |
| 规格： | 100.00 µg | 产品价格： | ￥1980.0 |
| 规格： | 500.00 µg | 产品价格： | ￥6480.0 |
重组人 TNF-alpha / TNFA 蛋白（产品说明）
蛋白名称：Human TNF-alpha / TNFA Protein
蛋白构建：A DNA sequence encoding the human TNF-α soluble form (NP_000585.2) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus.
表达宿主：E. coli
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性：Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.
蛋白内毒素：< 5 EU per mg of the protein as determined by the LAL method.
预测N端：Met
蛋白分子量：The recombinant human TNF-α consists of 158 amino acids and has a predicted molecular mass of 17.5 kDa which is also estimated by SDS-PAGE.
蛋白NP号：NP_000585.2
蛋白氨基酸序列：Val77-Leu233
蛋白标签：Native
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
蛋白名称：Human TNF-alpha / TNFA Protein
蛋白构建：A DNA sequence encoding the human TNF-α soluble form (NP_000585.2) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus.
表达宿主：E. coli
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性：Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.
蛋白内毒素：< 5 EU per mg of the protein as determined by the LAL method.
预测N端：Met
蛋白分子量：The recombinant human TNF-α consists of 158 amino acids and has a predicted molecular mass of 17.5 kDa which is also estimated by SDS-PAGE.
蛋白NP号：NP_000585.2
蛋白氨基酸序列：Val77-Leu233
蛋白标签：Native
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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- 内容
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文献和实验该产品被引用文献
1, Xu, Y; et al. Increased sCD200 Levels in Vitreous of Patients With Proliferative Diabetic Retinopathy and Its Correlation With VEGF and Proinflammatory Cytokines. Investigative ophthalmology & visual science, PMID: 26457542
2, Lin, X; et al. CYLD Promotes TNF-α-Induced Cell Necrosis Mediated by RIP-1 in Human Lung Cancer Cells. Mediators of inflammation, PMID: 27738385
3, Lei, H; et al. Human S100A7 Induces Mature Interleukin1α Expression by RAGE-p38 MAPK-Calpain1 Pathway in Psoriasis. PloS one, PMID: 28060905
4, Zhang, C; et al. Transcription factor NF-kappa B represses ANT1 transcription and leads to mitochondrial dysfunctions. Scientific reports, PMID: 28317877
5, Du, J; et al. Purification of target proteins from intracellular inclusions mediated by intein cleavable polyhydroxyalkanoate synthase fusions. Microbial cell factories, PMID: 29096637
6, Wu, Y; et al. Identification of Human Single-Domain Antibodies against SARS-CoV-2. Cell host & microbe, PMID: 32413276
7, Qi, G; et al. 1α,25-dihydroxyvitamin D3 promotes early osteogenic differentiation of PDLSCs and a 12-year follow-up case of early-onset vitamin D deficiency periodontitis. The Journal of steroid biochemistry and molecular biology, PMID: 33486080
8, Zeng, C; et al. SERINC proteins potentiate antiviral type I IFN production and proinflammatory signaling pathways. Science signaling, PMID: 34520227
9, Valionyte, E; et al. The caspase-6-p62 axis modulates p62 droplets based autophagy in a dominant-negative manner. Cell death and differentiation, PMID: 34862482
10, Luo, Z; et al. NicE-C efficiently reveals open chromatin-associated chromosome interactions at high resolution. Genome research, PMID: 35105668
11, Wang, ZW; et al. Repurposing of the FGFR inhibitor AZD4547 as a potent inhibitor of necroptosis by selectively targeting RIPK1. Acta pharmacologica Sinica, PMID: 36216899 1
2, Tang, Y; et al. Exosomal miR-27b-3p secreted by visceral adipocytes contributes to endothelial inflammation and atherogenesis. Cell reports, PMID: 36640325 1
3, Davé, E; et al. TRYBE®: an Fc-free antibody format with three monovalent targeting arms engineered for long in vivo half-life. mAbs, PMID: 36788124 1
4, Grasmeier, MK; et al. Surface plasmon resonance assays for the therapeutic drug monitoring of infliximab indicate clinical relevance of anti-infliximab antibody binding properties. Clinical chemistry and laboratory medicine, PMID: 36753693 1
5, Ran, XH; et al. TRIM5α recruits HDAC1 to p50 and Sp1 and promotes H3K9 deacetylation at the HIV-1 LTR. Nature communications, PMID: 37291137 1
6, Miao, Z; et al. [YKL-40 Promotes the Expression of Inflammatory Factors in Type Ⅱ Alveolar Epithelial Cell Model of A549 Cell Line]. Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, PMID: 37866952 1
7, Ryu, JJ; et al. A liquid crystal-based biosensor for sensitive detection of tumor necrosis factor-alpha. Mikrochimica acta, PMID: 38153588 1
8, Di Ianni, A; et al. Leveraging Cross-Linking Mass Spectrometry for Modeling Antibody-Antigen Complexes. Journal of proteome research, PMID: 38372774 1
9, Deng, B; et al. TNF and IFNγ-induced cell death requires IRF1 and ELAVL1 to promote CASP8 expression. The Journal of cell biology, PMID: 38319288
20, Hollis, WC; et al. Submicron immunoglobulin particles exhibit FcγRII-dependent toxicity linked to autophagy in TNFα-stimulated endothelial cells. Cellular and molecular life sciences : CMLS, PMID: 39212707
21, Ma, X; et al. Dual blockade of IL-17A and IL-36 pathways via a bispecific antibody exhibits enhanced anti-inflammatory potency. Frontiers in immunology, PMID: 39600699 2
2, Sun, Y; et al. Molecular mechanism through which Tripterygium hypoglaucum (Lévl.) Hutch alleviates psoriasis. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, PMID: 39504627 2
3, Awad, AM; et al. Cysteine Leukotriene Receptor Antagonist-Montelukast Effects on Diabetic Retinal Microvascular Endothelial Cells Curtail Autophagy. Investigative ophthalmology & visual science, PMID: 39504050 2
4, Zhang, L; et al. Detection of TNF-α using the established ab-MPs-CLIA. Talanta, PMID: 39637773 2
5, Ren, K; et al. Zhichuanling injection improves bronchial asthma by attenuation airway inflammation and epithelia-mesenchymal transition. Journal of ethnopharmacology, PMID: 40020793 2
6, Yu, T; et al. Glass fiber-interfaced CRISPR/Cas biosensing adaptable for diverse biomarker detection. Trends in biotechnology, PMID: 40447514 2
7, Li, T; et al. Non-canonical PRC1.1 licenses transcriptional response to enable Treg plasticity in immune adaptation. Molecular cell, PMID: 40527320 2
8, Zhang, P; et al. Endothelium-specific endoglin triggers astrocyte reactivity via extracellular vesicles in a mouse model of Alzheimer's disease. Molecular neurodegeneration, PMID: 40702549 2
9, Gao, X; et al. Vitamin D Alleviates Osteoarthritis Progression by Targeting Cartilage and Subchondral Bone via Myd88-TAK1-ERK Axis Suppression. Drug design, development and therapy, PMID: 40657039
30, Correa, NQ; et al. Presenilin 1 E280A mutation induces dysfunctional astrocytic phenotype in menstrual stromal-derived astrocyte-like cells. Cellular and molecular life sciences : CMLS, PMID: 41148249
31, Tao, Q; et al. Discovery of a 1H-Pyrazol-3-Amine Derivative as a Novel, Selective, and Orally Available RIPK1 Inhibitor for the Treatment of Inflammatory Disease. Journal of medicinal chemistry, PMID: 41077763
2, Lin, X; et al. CYLD Promotes TNF-α-Induced Cell Necrosis Mediated by RIP-1 in Human Lung Cancer Cells. Mediators of inflammation, PMID: 27738385
3, Lei, H; et al. Human S100A7 Induces Mature Interleukin1α Expression by RAGE-p38 MAPK-Calpain1 Pathway in Psoriasis. PloS one, PMID: 28060905
4, Zhang, C; et al. Transcription factor NF-kappa B represses ANT1 transcription and leads to mitochondrial dysfunctions. Scientific reports, PMID: 28317877
5, Du, J; et al. Purification of target proteins from intracellular inclusions mediated by intein cleavable polyhydroxyalkanoate synthase fusions. Microbial cell factories, PMID: 29096637
6, Wu, Y; et al. Identification of Human Single-Domain Antibodies against SARS-CoV-2. Cell host & microbe, PMID: 32413276
7, Qi, G; et al. 1α,25-dihydroxyvitamin D3 promotes early osteogenic differentiation of PDLSCs and a 12-year follow-up case of early-onset vitamin D deficiency periodontitis. The Journal of steroid biochemistry and molecular biology, PMID: 33486080
8, Zeng, C; et al. SERINC proteins potentiate antiviral type I IFN production and proinflammatory signaling pathways. Science signaling, PMID: 34520227
9, Valionyte, E; et al. The caspase-6-p62 axis modulates p62 droplets based autophagy in a dominant-negative manner. Cell death and differentiation, PMID: 34862482
10, Luo, Z; et al. NicE-C efficiently reveals open chromatin-associated chromosome interactions at high resolution. Genome research, PMID: 35105668
11, Wang, ZW; et al. Repurposing of the FGFR inhibitor AZD4547 as a potent inhibitor of necroptosis by selectively targeting RIPK1. Acta pharmacologica Sinica, PMID: 36216899 1
2, Tang, Y; et al. Exosomal miR-27b-3p secreted by visceral adipocytes contributes to endothelial inflammation and atherogenesis. Cell reports, PMID: 36640325 1
3, Davé, E; et al. TRYBE®: an Fc-free antibody format with three monovalent targeting arms engineered for long in vivo half-life. mAbs, PMID: 36788124 1
4, Grasmeier, MK; et al. Surface plasmon resonance assays for the therapeutic drug monitoring of infliximab indicate clinical relevance of anti-infliximab antibody binding properties. Clinical chemistry and laboratory medicine, PMID: 36753693 1
5, Ran, XH; et al. TRIM5α recruits HDAC1 to p50 and Sp1 and promotes H3K9 deacetylation at the HIV-1 LTR. Nature communications, PMID: 37291137 1
6, Miao, Z; et al. [YKL-40 Promotes the Expression of Inflammatory Factors in Type Ⅱ Alveolar Epithelial Cell Model of A549 Cell Line]. Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, PMID: 37866952 1
7, Ryu, JJ; et al. A liquid crystal-based biosensor for sensitive detection of tumor necrosis factor-alpha. Mikrochimica acta, PMID: 38153588 1
8, Di Ianni, A; et al. Leveraging Cross-Linking Mass Spectrometry for Modeling Antibody-Antigen Complexes. Journal of proteome research, PMID: 38372774 1
9, Deng, B; et al. TNF and IFNγ-induced cell death requires IRF1 and ELAVL1 to promote CASP8 expression. The Journal of cell biology, PMID: 38319288
20, Hollis, WC; et al. Submicron immunoglobulin particles exhibit FcγRII-dependent toxicity linked to autophagy in TNFα-stimulated endothelial cells. Cellular and molecular life sciences : CMLS, PMID: 39212707
21, Ma, X; et al. Dual blockade of IL-17A and IL-36 pathways via a bispecific antibody exhibits enhanced anti-inflammatory potency. Frontiers in immunology, PMID: 39600699 2
2, Sun, Y; et al. Molecular mechanism through which Tripterygium hypoglaucum (Lévl.) Hutch alleviates psoriasis. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, PMID: 39504627 2
3, Awad, AM; et al. Cysteine Leukotriene Receptor Antagonist-Montelukast Effects on Diabetic Retinal Microvascular Endothelial Cells Curtail Autophagy. Investigative ophthalmology & visual science, PMID: 39504050 2
4, Zhang, L; et al. Detection of TNF-α using the established ab-MPs-CLIA. Talanta, PMID: 39637773 2
5, Ren, K; et al. Zhichuanling injection improves bronchial asthma by attenuation airway inflammation and epithelia-mesenchymal transition. Journal of ethnopharmacology, PMID: 40020793 2
6, Yu, T; et al. Glass fiber-interfaced CRISPR/Cas biosensing adaptable for diverse biomarker detection. Trends in biotechnology, PMID: 40447514 2
7, Li, T; et al. Non-canonical PRC1.1 licenses transcriptional response to enable Treg plasticity in immune adaptation. Molecular cell, PMID: 40527320 2
8, Zhang, P; et al. Endothelium-specific endoglin triggers astrocyte reactivity via extracellular vesicles in a mouse model of Alzheimer's disease. Molecular neurodegeneration, PMID: 40702549 2
9, Gao, X; et al. Vitamin D Alleviates Osteoarthritis Progression by Targeting Cartilage and Subchondral Bone via Myd88-TAK1-ERK Axis Suppression. Drug design, development and therapy, PMID: 40657039
30, Correa, NQ; et al. Presenilin 1 E280A mutation induces dysfunctional astrocytic phenotype in menstrual stromal-derived astrocyte-like cells. Cellular and molecular life sciences : CMLS, PMID: 41148249
31, Tao, Q; et al. Discovery of a 1H-Pyrazol-3-Amine Derivative as a Novel, Selective, and Orally Available RIPK1 Inhibitor for the Treatment of Inflammatory Disease. Journal of medicinal chemistry, PMID: 41077763
相关实验
烟雨无心客 请问大家有知道炎症信号通路中TNFa的特异性抑制剂是什么的吗？如果有文献最好。万分感谢！ xiaoxiaoga Lenalidomide (Revlimid)Pomalidomide这两个是TNF-ALPHA的抑制剂， xiaoxiaoga References on Lenalidomide (Revlimid):[1] H Quach et al. Leukemia. 2010, 24:22–32[2] http://en.wikipedia.org/wiki
植物源重组人胰岛素生长因子融合蛋白（OsrIGF-1），胰岛素在无血清细胞培养中的最佳替代品。 IGFs家族由两种低分子多肽(IGF-Ⅰ、IGF-Ⅱ)、两类特异性受体及六种结合蛋白组成。IGF-Ⅰ是一个有70个氨基酸的单链碱性蛋白，分子量7649Da，耐热。IGFs的生物学功能不只局限于有丝分裂刺激作用，它们也能诱导分化或促进分化功能的表达。全新推出的植物源重组人类胰岛素生长因子-1融合蛋白（OsrIGF-1）在IGF-1的N末端融合了人血清白蛋白（HSA），HSA作为蛋白质保护剂增加了IGF
脂蛋白-X 【参考范围】免疫透射比浊法：0～90mg/L. 【影响因素】标本采集及分离与测定TCH的标本抽取相同。 【临床意义】1.Lp-X是出现在阻塞性黄疸患者血清中的一种异常的低密度脂蛋白，其生成与胆汁中的卵磷脂反流有关。因而利用其这种只在胆汁淤积患者血中出现的特殊性，可以对黄疸或肝内、肝外阻塞进行一定程度的鉴别医学教|育网搜集整理。一般认为肝外胆道的梗阻比肝内的阻塞引起的胆汁淤积程度要更为严重，其参考的鉴别值为2O00mg/L. 2.在急性肝炎、肝癌、胆道
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重组人 TNF-alpha / TNFA蛋白
￥580 - 10980
