重组人TNF-alpha / TNFA蛋白产品图

重组人TNF-alpha / TNFA蛋白

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  • ¥169 - 9800
  • Sino Biological已认证
  • 北京
  • 10602-HNAE
  • 2026年08月31日
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    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 保存条件

      -20℃ to -80℃

    • 保质期

      12个月

    • 英文名

      Human TNF-alpha / TNFA Protein

    • 库存

      99

    • 供应商

      北京义翘神州科技股份有限公司

    • 规格

      10 µg/50 µg/100 µg/500 µg/1 mg

    规格:10 µg产品价格:¥169.0
    规格:50 µg产品价格:¥1380.0
    规格:100 µg产品价格:¥1980.0
    规格:500 µg产品价格:¥6480.0
    规格:1 mg产品价格:¥9800.0
    重组人TNF-alpha / TNFA蛋白(产品说明)
    蛋白名称:重组人TNF-alpha / TNFA蛋白
    蛋白构建:A DNA sequence encoding the human TNF-α soluble form (NP_000585.2) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus.
    表达宿主:E. coli
    蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 95% as determined by SEC-MALS.
    生物活性:1.Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL. 2.Captured Anti-TNFa (Research Grade Adalim*mab Biosimilar) on Anti-human IgG Fc via CM5 Chip can TNF-α (Cat.No.10602-HNAE) with an affinity constant of 0.2843 nM as determined in a SPR assay (Biacore T200)(Routinely tested). 3.Anti-TNFa (Research Grade Adalim*mab Biosimilar) captured on Protein A chip can bind Recombinant Human TNF-alpha Protein (Cat. No. 10602-HNAE) with an affinity constant of 0.12 nM as determined in an SPR assay (Biacore 8K) (Routinely tested). 4.Immobilized Recombinant Human TNF-alpha / TNFA Protein (Cat: 10602-HNAE) at 1 μg/mL (100 μL/well) can bind Anti-TNFa (Research Grade Adalim*mab Biosimilar), the EC50 is 1.5-5.0 ng/mL (Routinely tested). 5.Immobilized Recombinant Human TNF-alpha / TNFA Protein (Cat: 10602-HNAE) at 1 μg/mL (100 μL/well) can bind Anti-TNFa (Research Grade Inflix*mab Biosimilar), the EC50 is 1.5-5.0 ng/mL (Routinely tested). 6.Immobilized Recombinant Human TNF-alpha / TNFA Protein (Cat: 10602-HNAE) at 1 μg/mL (100 μL/well) can bind Anti-TNFa (Research Grade Inflix*mab Biosimilar), the EC50 is 1.5-5.0 ng/mL (Routinely tested). 7.Immobilized Recombinant Human TNF-alpha / TNFA Protein (Cat: 10602-HNAE) at 1 μg/mL (100 μL/well) can bind Anti-TNFa (Research Grade Golim*mab Biosimilar), the EC50 is 2.0-6.0 ng/mL (Routinely tested). 8.Immobilized Recombinant Human TNF-alpha / TNFA Protein (Cat: 10602-HNAE) at 2 μg/mL (100 μL/well) can bind Recombinant Human TNFRSF1A Protein (Fc Tag) (Cat: 10872-H02H), the EC50 is 5.6-17.0 ng/mL (QC tested). 9.Immobilized Recombinant Human TNF-alpha / TNFA Protein (Cat: 10602-HNAE) at 5 μg/mL (100 μL/well) can bind Recombinant Human TNFR2 / CD120b / TNFRSF1B Protein (ECD, His & Fc Tag) (Cat: 10417-H03H), the EC50 is 5.2-15.5 ng/mL (Routinely tested).
    内毒素:< 5 EU per mg of the protein as determined by the LAL method.
    预测N端:Met
    蛋白分子量:The recombinant human TNF-α consists of 158 amino acids and has a predicted molecular mass of 17.5 kDa which is also estimated by SDS-PAGE. The molecular weight of this protein is around 50.3 kDa verified by SEC-MALS.
    蛋白NP号:NP_000585.2
    蛋白氨基酸序列:Val77-Leu233

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    图标文献和实验
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    [9]Imatinib and methazolamide ameliorate COVID-19-induced metabolic complications via elevating ACE2 enzymatic activity and inhibiting viral entry
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