- ￥580 - 10980
- Sino Biological
- 北京
- 10602-HNAE
- 2025年08月12日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human TNF-alpha / TNFA Protein
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1.00 mg/10.00 µg/50.00 µg/100.00 µg/500.00 µg
| 规格： | 1.00 mg | 产品价格： | ￥10980.0 |
|---|---|---|---|
| 规格： | 10.00 µg | 产品价格： | ￥580.0 |
| 规格： | 50.00 µg | 产品价格： | ￥1380.0 |
| 规格： | 100.00 µg | 产品价格： | ￥1980.0 |
| 规格： | 500.00 µg | 产品价格： | ￥6480.0 |
重组人 TNF-alpha / TNFA 蛋白（产品说明）
蛋白名称：Human TNF-alpha / TNFA Protein
蛋白构建：A DNA sequence encoding the human TNF-α soluble form (NP_000585.2) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus.
表达宿主：E. coli
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性：Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.
蛋白内毒素：< 5 EU per mg of the protein as determined by the LAL method.
预测N端：Met
蛋白分子量：The recombinant human TNF-α consists of 158 amino acids and has a predicted molecular mass of 17.5 kDa which is also estimated by SDS-PAGE.
蛋白NP号：NP_000585.2
蛋白氨基酸序列：Val77-Leu233
蛋白标签：Native
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
蛋白名称：Human TNF-alpha / TNFA Protein
蛋白构建：A DNA sequence encoding the human TNF-α soluble form (NP_000585.2) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus.
表达宿主：E. coli
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性：Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.
蛋白内毒素：< 5 EU per mg of the protein as determined by the LAL method.
预测N端：Met
蛋白分子量：The recombinant human TNF-α consists of 158 amino acids and has a predicted molecular mass of 17.5 kDa which is also estimated by SDS-PAGE.
蛋白NP号：NP_000585.2
蛋白氨基酸序列：Val77-Leu233
蛋白标签：Native
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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- 作者
- 内容
- 询问日期
文献和实验该产品被引用文献
1, Xu Y, et al. Increased sCD200 Levels in Vitreous of Patients With Proliferative Diabetic Retinopathy and Its Correlation With VEGF and Proinflammatory Cytokines.Investigative ophthalmology & visual science, PubMed ID: 26457542
2, Lin X, et al. CYLD Promotes TNF-α-Induced Cell Necrosis Mediated by RIP-1 in Human Lung Cancer Cells.Mediators of inflammation, PubMed ID: 27738385
3, Lei H, et al. Human S100A7 Induces Mature Interleukin1α Expression by RAGE-p38 MAPK-Calpain1 Pathway in Psoriasis.PloS one, PubMed ID: 28060905
4, Zhang C, et al. Transcription factor NF-kappa B represses ANT1 transcription and leads to mitochondrial dysfunctions.Scientific reports, PubMed ID: 28317877
5, Du J, et al. Purification of target proteins from intracellular inclusions mediated by intein cleavable polyhydroxyalkanoate synthase fusions.Microbial cell factories, PubMed ID: 29096637
6, Wu Y, et al. Identification of Human Single-Domain Antibodies against SARS-CoV-2.Cell host & microbe, PubMed ID: 32413276
7, Qi G, et al. 1α,25-dihydroxyvitamin D3 promotes early osteogenic differentiation of PDLSCs and a 12-year follow-up case of early-onset vitamin D deficiency periodontitis.The Journal of steroid biochemistry and molecular biology, PubMed ID: 33486080
8, Zeng C, et al. SERINC proteins potentiate antiviral type I IFN production and proinflammatory signaling pathways.Science signaling, PubMed ID: 34520227
9, Valionyte E, et al. The caspase-6-p62 axis modulates p62 droplets based autophagy in a dominant-negative manner.Cell death and differentiation, PubMed ID: 34862482
10, Luo Z, et al. NicE-C efficiently reveals open chromatin-associated chromosome interactions at high resolution.Genome research, PubMed ID: 35105668
11, Wang ZW, et al. Repurposing of the FGFR inhibitor AZD4547 as a potent inhibitor of necroptosis by selectively targeting RIPK1.Acta pharmacologica Sinica, PubMed ID: 36216899
12, Tang Y, et al. Exosomal miR-27b-3p secreted by visceral adipocytes contributes to endothelial inflammation and atherogenesis.Cell reports, PubMed ID: 36640325
13, Davé E, et al. TRYBE®: an Fc-free antibody format with three monovalent targeting arms engineered for long in vivo half-life.mAbs, PubMed ID: 36788124
14, Grasmeier MK, et al. Surface plasmon resonance assays for the therapeutic drug monitoring of infliximab indicate clinical relevance of anti-infliximab antibody binding properties.Clinical chemistry and laboratory medicine, PubMed ID: 36753693
15, Ran XH, et al. TRIM5α recruits HDAC1 to p50 and Sp1 and promotes H3K9 deacetylation at the HIV-1 LTR.Nature communications, PubMed ID: 37291137
16, Miao Z, et al. [YKL-40 Promotes the Expression of Inflammatory Factors in Type Ⅱ Alveolar Epithelial Cell Model of A549 Cell Line].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, PubMed ID: 37866952
17, Ryu JJ, et al. A liquid crystal-based biosensor for sensitive detection of tumor necrosis factor-alpha.Mikrochimica acta, PubMed ID: 38153588
18, Di Ianni A, et al. Leveraging Cross-Linking Mass Spectrometry for Modeling Antibody-Antigen Complexes.Journal of proteome research, PubMed ID: 38372774
19, Deng B, et al. TNF and IFNγ-induced cell death requires IRF1 and ELAVL1 to promote CASP8 expression.The Journal of cell biology, PubMed ID: 38319288
20, Hollis WC, et al. Submicron immunoglobulin particles exhibit FcγRII-dependent toxicity linked to autophagy in TNFα-stimulated endothelial cells.Cellular and molecular life sciences : CMLS, PubMed ID: 39212707
2, Lin X, et al. CYLD Promotes TNF-α-Induced Cell Necrosis Mediated by RIP-1 in Human Lung Cancer Cells.Mediators of inflammation, PubMed ID: 27738385
3, Lei H, et al. Human S100A7 Induces Mature Interleukin1α Expression by RAGE-p38 MAPK-Calpain1 Pathway in Psoriasis.PloS one, PubMed ID: 28060905
4, Zhang C, et al. Transcription factor NF-kappa B represses ANT1 transcription and leads to mitochondrial dysfunctions.Scientific reports, PubMed ID: 28317877
5, Du J, et al. Purification of target proteins from intracellular inclusions mediated by intein cleavable polyhydroxyalkanoate synthase fusions.Microbial cell factories, PubMed ID: 29096637
6, Wu Y, et al. Identification of Human Single-Domain Antibodies against SARS-CoV-2.Cell host & microbe, PubMed ID: 32413276
7, Qi G, et al. 1α,25-dihydroxyvitamin D3 promotes early osteogenic differentiation of PDLSCs and a 12-year follow-up case of early-onset vitamin D deficiency periodontitis.The Journal of steroid biochemistry and molecular biology, PubMed ID: 33486080
8, Zeng C, et al. SERINC proteins potentiate antiviral type I IFN production and proinflammatory signaling pathways.Science signaling, PubMed ID: 34520227
9, Valionyte E, et al. The caspase-6-p62 axis modulates p62 droplets based autophagy in a dominant-negative manner.Cell death and differentiation, PubMed ID: 34862482
10, Luo Z, et al. NicE-C efficiently reveals open chromatin-associated chromosome interactions at high resolution.Genome research, PubMed ID: 35105668
11, Wang ZW, et al. Repurposing of the FGFR inhibitor AZD4547 as a potent inhibitor of necroptosis by selectively targeting RIPK1.Acta pharmacologica Sinica, PubMed ID: 36216899
12, Tang Y, et al. Exosomal miR-27b-3p secreted by visceral adipocytes contributes to endothelial inflammation and atherogenesis.Cell reports, PubMed ID: 36640325
13, Davé E, et al. TRYBE®: an Fc-free antibody format with three monovalent targeting arms engineered for long in vivo half-life.mAbs, PubMed ID: 36788124
14, Grasmeier MK, et al. Surface plasmon resonance assays for the therapeutic drug monitoring of infliximab indicate clinical relevance of anti-infliximab antibody binding properties.Clinical chemistry and laboratory medicine, PubMed ID: 36753693
15, Ran XH, et al. TRIM5α recruits HDAC1 to p50 and Sp1 and promotes H3K9 deacetylation at the HIV-1 LTR.Nature communications, PubMed ID: 37291137
16, Miao Z, et al. [YKL-40 Promotes the Expression of Inflammatory Factors in Type Ⅱ Alveolar Epithelial Cell Model of A549 Cell Line].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, PubMed ID: 37866952
17, Ryu JJ, et al. A liquid crystal-based biosensor for sensitive detection of tumor necrosis factor-alpha.Mikrochimica acta, PubMed ID: 38153588
18, Di Ianni A, et al. Leveraging Cross-Linking Mass Spectrometry for Modeling Antibody-Antigen Complexes.Journal of proteome research, PubMed ID: 38372774
19, Deng B, et al. TNF and IFNγ-induced cell death requires IRF1 and ELAVL1 to promote CASP8 expression.The Journal of cell biology, PubMed ID: 38319288
20, Hollis WC, et al. Submicron immunoglobulin particles exhibit FcγRII-dependent toxicity linked to autophagy in TNFα-stimulated endothelial cells.Cellular and molecular life sciences : CMLS, PubMed ID: 39212707
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TNF-alpha重组蛋白|Recombinant Human TNF-alpha / TNFA Protein
￥580 - 10980
