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- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Human TNF-alpha / TNFA Protein
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
10 µg/50 µg/100 µg/500 µg/1 mg
| 规格: | 10 µg | 产品价格: | ¥169.0 |
|---|---|---|---|
| 规格: | 50 µg | 产品价格: | ¥1380.0 |
| 规格: | 100 µg | 产品价格: | ¥1980.0 |
| 规格: | 500 µg | 产品价格: | ¥6480.0 |
| 规格: | 1 mg | 产品价格: | ¥9800.0 |
蛋白名称:重组人TNF-alpha / TNFA蛋白
蛋白构建:A DNA sequence encoding the human TNF-α soluble form (NP_000585.2) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus.
表达宿主:E. coli
蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 95% as determined by SEC-MALS.
生物活性:1.Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL. 2.Captured Anti-TNFa (Research Grade Adalim*mab Biosimilar) on Anti-human IgG Fc via CM5 Chip can TNF-α (Cat.No.10602-HNAE) with an affinity constant of 0.2843 nM as determined in a SPR assay (Biacore T200)(Routinely tested). 3.Anti-TNFa (Research Grade Adalim*mab Biosimilar) captured on Protein A chip can bind Recombinant Human TNF-alpha Protein (Cat. No. 10602-HNAE) with an affinity constant of 0.12 nM as determined in an SPR assay (Biacore 8K) (Routinely tested). 4.Immobilized Recombinant Human TNF-alpha / TNFA Protein (Cat: 10602-HNAE) at 1 μg/mL (100 μL/well) can bind Anti-TNFa (Research Grade Adalim*mab Biosimilar), the EC50 is 1.5-5.0 ng/mL (Routinely tested). 5.Immobilized Recombinant Human TNF-alpha / TNFA Protein (Cat: 10602-HNAE) at 1 μg/mL (100 μL/well) can bind Anti-TNFa (Research Grade Inflix*mab Biosimilar), the EC50 is 1.5-5.0 ng/mL (Routinely tested). 6.Immobilized Recombinant Human TNF-alpha / TNFA Protein (Cat: 10602-HNAE) at 1 μg/mL (100 μL/well) can bind Anti-TNFa (Research Grade Inflix*mab Biosimilar), the EC50 is 1.5-5.0 ng/mL (Routinely tested). 7.Immobilized Recombinant Human TNF-alpha / TNFA Protein (Cat: 10602-HNAE) at 1 μg/mL (100 μL/well) can bind Anti-TNFa (Research Grade Golim*mab Biosimilar), the EC50 is 2.0-6.0 ng/mL (Routinely tested). 8.Immobilized Recombinant Human TNF-alpha / TNFA Protein (Cat: 10602-HNAE) at 2 μg/mL (100 μL/well) can bind Recombinant Human TNFRSF1A Protein (Fc Tag) (Cat: 10872-H02H), the EC50 is 5.6-17.0 ng/mL (QC tested). 9.Immobilized Recombinant Human TNF-alpha / TNFA Protein (Cat: 10602-HNAE) at 5 μg/mL (100 μL/well) can bind Recombinant Human TNFR2 / CD120b / TNFRSF1B Protein (ECD, His & Fc Tag) (Cat: 10417-H03H), the EC50 is 5.2-15.5 ng/mL (Routinely tested).
内毒素:< 5 EU per mg of the protein as determined by the LAL method.
预测N端:Met
蛋白分子量:The recombinant human TNF-α consists of 158 amino acids and has a predicted molecular mass of 17.5 kDa which is also estimated by SDS-PAGE. The molecular weight of this protein is around 50.3 kDa verified by SEC-MALS.
蛋白NP号:NP_000585.2
蛋白氨基酸序列:Val77-Leu233
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植物源重组人胰岛素生长因子融合蛋白(OsrIGF-1),胰岛素在无血清细胞培养中的最佳替代品。 IGFs家族由两种低分子多肽(IGF-Ⅰ、IGF-Ⅱ)、两类特异性受体及六种结合蛋白组成。IGF-Ⅰ是一个有70个氨基酸的单链碱性蛋白,分子量7649Da,耐热。IGFs的生物学功能不只局限于有丝分裂刺激作用,它们也能诱导分化或促进分化功能的表达。全新推出的植物源重组人类胰岛素生长因子-1融合蛋白(OsrIGF-1)在IGF-1的N末端融合了人血清白蛋白(HSA),HSA作为蛋白质保护剂增加了IGF
脂蛋白-X 【参考范围】免疫透射比浊法:0~90mg/L. 【影响因素】标本采集及分离与测定TCH的标本抽取相同。 【临床意义】1.Lp-X是出现在阻塞性黄疸患者血清中的一种异常的低密度脂蛋白,其生成与胆汁中的卵磷脂反流有关。因而利用其这种只在胆汁淤积患者血中出现的特殊性,可以对黄疸或肝内、肝外阻塞进行一定程度的鉴别医学教|育网搜集整理。一般认为肝外胆道的梗阻比肝内的阻塞引起的胆汁淤积程度要更为严重,其参考的鉴别值为2O00mg/L. 2.在急性肝炎、肝癌、胆道
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