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50 µg
蛋白名称:VEGFA蛋白, VEGFA protein
蛋白构建:A DNA sequence encoding the human VEGF 183 isoform (P15692-3) (Met1-Arg209) was expressed.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:> 95 % as determined by SDS-PAGE.
蛋白活性:Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 50-210 ng/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Ala 27
蛋白分子量:The recombinant human VEGF 183 isoform consists of 183 amino acids and has a predicted molecular mass of 21 kDa.
蛋白NP号:P15692-3
蛋白氨基酸序列:Met1-Arg209
蛋白标签:Native
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Cytokine induced angiogenesis in chick embryos.
Objective: The purpose of this experiment was to explore the effects of the growth factors bFGF and VEGF on blood vessel formation within the chorioallantoic membrane (CAM) of chick embryos. Background Information:
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 and Total-p38 antibodies (C). Cell-Based Elisa将小鼠巨噬细胞4/4细胞种在96孔板内,每个孔种5 x 104 cells/cm2细胞。细胞用不同浓度的anisomycin刺激,我们来监测P38 MAPK和JNK两种蛋白的变化。按照试剂盒的实验步骤,分别固定,打孔,猝灭,封闭,然后一抗孵育,二抗孵育,最后显色,用常规的酶标仪读取数值。最后数据再和试剂盒提供的结晶紫核染料结果做纠正,最后得出以上数据。Quantitative
坏死因子(Tumornecrosisfactor,TNF)根据其产生来源和结构不同,可分为TNF-α和TNF-β两类,前者由单核-巨噬细胞产生,后者由活化T细胞产生,又名淋巴毒素。TNF除具有杀伤肿瘤细胞外,还有免疫调节、参与发热和炎症的发生。 5,转化生长因子-β家族(Transforminggrowthfactor-βfamily,TGF-βfamily)主要包括TGF-β1、TGF-β2、TGF-β3、TGFβ1β2以及骨形成蛋白(BMP)等。 6.趋化因子家族(Chemokinefamily)包括两个亚族:(1)C-X-C
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