- ￥2310 - 15990
- Sino Biological
- 北京
- 10694-H08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human EpCAM / TROP-1 / TACSTD1 Protein (ECD, His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1.00 mg/50.00 µg/100.00 µg
| 规格： | 1.00 mg | 产品价格： | ￥15990.0 |
|---|---|---|---|
| 规格： | 50.00 µg | 产品价格： | ￥2310.0 |
| 规格： | 100.00 µg | 产品价格： | ￥2460.0 |
蛋白名称：Human EpCAM / TROP-1 / TACSTD1 Protein (ECD, His Tag)
蛋白构建：A DNA sequence encoding the extracellular domain (Met1-Lys265) of human EpCAM (NP_002345.1) was fused with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 96 % as determined by SDS-PAGE
蛋白活性：Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When cells are added to EpCAM-His coated plates (1.25μg/mL, 100μL/well), approximately >40% will adhere specifically after 30 minutes at 37℃.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gln 24
蛋白分子量：The recombinant human EpCAM consists of 253 amino acids after removal of the signal peptide and has a calculated molecular mass of 28.84 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human EpCAM is approximately 36.5 kDa due to glycosylation.
蛋白NP号：NP_002345.1
蛋白氨基酸序列：Met1-Lys265
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Bell DR, et al. In silico design and validation of high-affinity RNA aptamers targeting epithelial cellular adhesion molecule dimers.Proceedings of the National Academy of Sciences of the United States of America, PubMed ID: 32234785
3, Wu X, et al. Detection of Hepatocellular Carcinoma in an Orthotopic Patient-Derived Xenograft with an Epithelial Cell Adhesion Molecule-Specific Peptide.Cancers, PubMed ID: 39199591
.7. Wang, X. et al. 2011. Study of two G-protein coupled receptor variants of human trace amine-associated receptor 5. Sci Rep1: 102 (DOI:10.1038/srep00102).8. Wang, X. and Zhang, S. 2011. Production of a bioengineered G-protein coupled receptor of human
100-18B Human FGF-basic，10mM Tri(pH 8.0)用于溶解100-25 Human FGF-8。 关于PeproTech公司重组细胞因子和蛋白溶解的具体方法和注意事项请见：http://www.biomart.cn/experiment/430/488/490/492/31321.htm PeproTech公司简介： PeproTech Inc. 创立于1988年，始终致力于为生命科学研究开发重组蛋白及相关产品
X-100或脱氧胆酸钠）和低浓度变性剂（2mol/L尿素或盐酸胍等）洗涤除去脂类和膜蛋白，这一步很重要，否则会导致包涵体溶解和复性的过程中重组蛋白质的降解[6、7、8]。 包涵体的溶解必须用很强的变性剂，如8mol/L尿素、6~8mol/L盐酸胍，通过离子间的相互作用破坏包涵体蛋白间的氢键而增溶蛋白。其中尿素的增溶效果稍差，异氰盐酸胍最强；去污剂，如SDS[7]，可以破坏蛋白内的疏水键，可以增溶几乎所有的蛋白，但由于无法彻底去除而不允许用在制药行业中；酸，如70%甲酸[9]，可以破坏
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