重组人 EpCAM / TROP-1 / TACSTD1 蛋白 (ECD, His标签)(产品说明)
蛋白名称:Human EpCAM / TROP-1 / TACSTD1 Protein (ECD, His Tag)
蛋白构建:A DNA sequence encoding the extracellular domain (Met1-Lys265) of human EpCAM (NP_002345.1) was fused with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 96 % as determined by SDS-PAGE
蛋白活性:Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When cells are added to EpCAM-His coated plates (1.25μg/mL, 100μL/well), approximately >40% will adhere specifically after 30 minutes at 37℃.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Gln 24
蛋白分子量:The recombinant human EpCAM consists of 253 amino acids after removal of the signal peptide and has a calculated molecular mass of 28.84 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human EpCAM is approximately 36.5 kDa due to glycosylation.
蛋白NP号:NP_002345.1
蛋白氨基酸序列:Met1-Lys265
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.