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- Sino Biological
- 10005-HCCH
- 2025年08月12日
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- 文献和实验
- 技术资料
- 规格：
100 µg
蛋白名称：Nectin-2蛋白, Nectin-2 protein
蛋白构建：The mature form of recombinant human CD112 (NP_002847.1) extracellular domain (Met 1-Leu 360) with quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE.
蛋白活性：Measured by its binding ability in a functional ELISA. Immobilized human CD112 (Cat: 10005-HCCH) at 10 μg/ml (100 μl/well) can bind human DNAM1-Fch (Cat: 10565-H03H) with a linear range of 1.25-10 μg/ml.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gln 32
蛋白分子量：The recombinant human CD112 comprises 335 amino acids and has a calculated molecular mass of 36.2 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 48 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号：NP_002847.1
蛋白氨基酸序列：Met1-Leu360
蛋白标签：C-cleavage
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验一、简介：1 细胞质膜资料 1895年,Overton从研究细胞透性得出"细胞膜由连续的脂类物质组成"。 1925年 Gorter&Grendel:用脂单分子膜技术测定细胞膜中脂分子的总面积，提出："细胞膜是由双层脂分子组成"。 1935年 Danielli&Davson：从测定膜的表面张力得出细胞膜的"三明治结构模型"，即蛋白质－脂-蛋白质。 1959年 Robertson:用电镜观察生物膜提出
蛋白分离纯化概述（An overview of protein isolation）
Isolating a protein may be compared to playing a game of golf. In golf, the player is faced with a series of problems, each unique and yet similar to problems previously encountered. In facing each problem the player must analyse
In order to carry out detailed studies of a protein (structure,enzyme activity, amino acid sequence, etc) it has to be very pure How is a single protein isolated from all the other proteins in the cell
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