- 询价
- Thermo scientific -fermentas
- SM1823
- 2025年10月24日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS |
| 2X RNA Loading Dye | ||||
| SM1823 | 50 applic. | 1.00 ml | SM1823 |
- Features
-
- 条带窄且亮度一致。
- 条带大小和含量容易记忆。
- 条带浓度预先设置好，可用于RNA条带的粗略定量。
- 配套提供2X RNA Loading Dye。
- -20°C可稳定保存6个月。
- Applications
-
- 变性胶和非变性胶中RNA分子大小鉴定和定量。
- Northern杂交。
RiboRuler™ High Range RNA Ladder, ready-to-use, 是由8条分子量大小200 - 6000 b的单链RNA转录子组成的混合物。
RiboRuler™ High Range RNA ladders, ready-to-use, 由层析纯化的转录子组成，无NTPs和RNA降解产物污染。每个RNA转录子的浓度均由分光光度计测定。为了减少使用时的污染机率，建议将Ladders分装后保存。
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文献和实验(2 μl) RNA ladder with 5 μg anchored Oligo(dT)20 primer at 70°C for 5 minutes. Keep on ice. 2) Combine the RNA-oligo dT complex from Step 1 with 1X 1st strand cDNA synthesis buffer supplemented with 10 mM DTT, 500 μM dNTP, 2 U RNaseOUT™
Separation and Size Determination of DNA over a 10,200 kbp Range
7 ). Here conditions are described for the separation of DNA in the l0–,200-kbp size range by CHEF. CHEF resolution in this range exceeds that of many commonly used size standards. Production and use of a size standard giving a 10-kbp ladder in the l0–,200-kbp range
is cDNA concentration 200 ng/ul too high for qRT-PCR-Rea
like to increase the concentration of cDNA to 200 ng/ul but am wondering if this can cause any problems. I use SYBR green. Also, my water blank with reference gene(18S) has a CT value of 32.0. I have run this with DEPC water, and two different bottles
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