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- Thermo scientific -fermentas
- SM1273
- 2025年10月21日
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MassRuler™ Express LR Forward DNA Ladder, ready-to-use
| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS |
| 6X MassRuler™ DNA Loading Dye | ||||
| SM1263 | 50-200 applic. (28 ng/µl) | 1.00 ml | SM1263 |
MassRuler™ Express LR Reverse DNA Ladder, ready-to-use
| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS |
| 6X MassRuler™ DNA Loading Dye | ||||
| SM1273 | 50-200 applic. (28 ng/µl) | 1.00 ml | SM1273 |
- Features
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- 精确测定DNA大小和含量。Ladder中各条带含量经分光光度计测定。
- 适合在不同电泳条件下通过短距离电泳快速、可靠地定量DNA条带。
- 条带窄。
- 条带大小和含量容易记忆。
- 即用型，与6X MassRuler™ DNA Loading Dye预先混合，可直接上样和室温保存。
- 配套提供样本DNA上样染料。
Ladder中每个条带的亮度均经标准品校准，确保每个条带的精确用量。该类分子量标准是层析纯化的DNA片段的混合物。
-20°C可保存更长时间。
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文献和实验2’ 1 cycle 94 oC 45”, 57 oC 1’, 70 oC 1’ 25 cycles 70 oC 5’ 1 cycle Analyze 10 ul PCR product on a 4-20% Novex gel along with 1kb ladder. You should see strong smear (predominantly in 100-700bp range) in all the +ligation dilutions but none of the �
by adding 1/10 volume of 3 M sodium acetate and 2X volume of 100% ethanol. 6. Centrifuge at maximum speed (14,000 rpm in a microcentrifuge) for 5 minutes at room temperature to pellet the DNA. 7. Remove the ethanol, rinse the pellet with 80% ethanol
in at least one plasmid without mutations. Materials Thermocycler PCR tubes Primers Phusion PCR Mastermix(or another high fidelity polymerase mastermix) BioBrick template DNA (miniprepped plasmid diluted 1:1000 in water) Qiagen PCR
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