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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
大量
|
| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS |
| 2X RNA Loading Dye | ||||
| SM1821 | 100 µl | 1.00 ml | SM1821 |
- Features
-
- 条带窄且亮度一致。
- 条带大小和含量容易记忆。
- 条带浓度预先设置好,可用于RNA条带的粗略定量。
- 配套提供2X RNA Loading Dye。
- -20°C可稳定保存6个月。
- Applications
-
- 变性胶和非变性胶中RNA分子大小鉴定和定量。
- Northern杂交。
RiboRuler™ High Range RNA Ladder 是由8条分子量大小200 - 6000 b的单链RNA转录子组成的混合物。
RiboRuler™ High Range RNA ladders由层析纯化的转录子组成,无NTPs和RNA降解产物污染。每个RNA转录子的浓度均由分光光度计测定。为了减少使用时的污染机率,建议将Ladders分装后保存。
RiboRuler™ High Range RNA ladders 可用 于T4 Polynucleotide Kinase (#EK0031)催化的末端标记(见操作方法)和Northern杂交等应用。
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文献和实验is cDNA concentration 200 ng/ul too high for qRT-PCR-Rea
...I try to get my stuff in the 18-26 range before I consider it to be good reliable data with high reproducibility. anyone else have thoughts on these high Ct's? -aimikins- Of course, I have "heard" of primer dimer. Not sure
, 4.8 kb in length, consistently migrates just ahead of the 4 kb peak in the RNA 6000 Ladder. This is likely due to the highly structured nature of 23S and 28S rRNAs. Although this assay has a broad linear range (~25 ng500 ng) overloading
Eukaryotic messenger RNA (mRNA) can be separated from the other RNA species in a total RNA preparation by affinity chromatography by virtue of the presence of a polyadenylic acid “tail,” 20–25 bases long, at the 3′ end of the molecule
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