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- 技术资料
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大量
|
| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| R1151 | 5 x 1 ml | R1151 |
6X DNA Loading Dye & SDS Solution
示踪染料电泳
- Features
-
- 1% SDS阻止DNA蛋白相互作用,防止含有粘性末端的DNA分子退火形成额外条带。
- 加入100 mM EDTA可立即终止金属依赖性酶促反应。
- Applications
-
- 高蛋白含量DNA样本分析。
- 动力学实验。
- DNA限制酶切割、连接和去磷酸化后琼脂糖凝胶分析。
- 0.03%溴酚蓝,
- 0.03%二甲苯青FF,
- 60%甘油,
- 1% SDS,
- 100 mM EDTA (pH 7.6, 用Tris调节)
-20°C可长期保存。
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文献和实验Clonality - X Chromosome Inactivation Assay
) 10X TBE (1 M Tris, 0.9 M Boric Acid, and 0.01 M EDTA) (Life Technologies) Loading dye (95% formamide, 20 mM EDTA, 0.05% bromophenol blue, 0.05% xylene cyanol) 2. Methods TIP: Investigators must be especially careful when using this methodology
8.8 (resolving gel) 1.0 M Tris, pH 6.8 (stacking gel) 5x SDS Running Buffer (1 L) Tris 15 g Glycine 72 g SDS 5 g Coomassie Blue Stain 10% (v/v) acetic acid 0.006% (w/v) Coomassie Blue dye 90% ddH2O Isopropanol Fixing Solution 10
is separated from the DNA by loading the solution onto an equilibrated ion exchange column. The A260 containing fractions are pooled, diluted, and ethanol precipitated, and the final DNA pellet is resuspended in buffer and assayed by restriction digestion
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