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- Thermo scientific -fermentas
- SM1293
- 2025年10月21日
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- 详细信息
- 文献和实验
- 技术资料
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MassRuler™ Express Forward DNA Ladder Mix, ready-to-use
| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS |
| 6X MassRuler™ DNA Loading Dye | ||||
| SM1283 | 50-200 applic. (56.5 ng/µl) | 1.00 ml | SM1283 |
MassRuler™ Express Reverse DNA Ladder Mix, ready-to-use
| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS |
| 6X MassRuler™ DNA Loading Dye | ||||
| SM1293 | 50-200 applic. (56.5 ng/µl) | 1.00 ml | SM1293 |
- Features
-
- 精确测定DNA大小和含量。Ladder中各条带含量经分光光度计测定。
- 适合在不同电泳条件下通过短距离电泳快速、可靠地定量DNA条带。
- 条带窄。
- 条带大小和含量容易记忆。
- 即用型，与6X MassRuler™ DNA Loading Dye预先混合，可直接上样和室温保存。
- 配套提供样本DNA上样染料。
Ladder中每个条带的亮度均经标准品校准，确保每个条带的精确用量。该类分子量标准是层析纯化的DNA片段的混合物。
-20°C可保存更长时间。
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文献和实验Incubate at 42 ° for 30-60 min for reverse transcription to proceed. For higher specificity and better resolution of RNA secondary structures, one can use up to 50 °C with Superscript II™ enzyme and up to 60 °C with the Superscript III™ enzyme. DMSO added
by adding 1/10 volume of 3 M sodium acetate and 2X volume of 100% ethanol. 6. Centrifuge at maximum speed (14,000 rpm in a microcentrifuge) for 5 minutes at room temperature to pellet the DNA. 7. Remove the ethanol, rinse the pellet with 80% ethanol
Safety Data Sheet regarding safe handling and use of these materials.A.Preparation of ddA,ddC,ddG,ddT termination mixes (Estimated Time 15-30 min.)**Only one set of termination mixes are required to sequence up to 10 DNA templates—DO NOT set
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