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- 技术资料
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大量
|
| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| K0512 | for 100 preps. one preparation is sufficient for: 200 µl of whole blood, 25-30 mg of mammalian tissue, 50-100 mg of plant tissue, 0.4-0.6x106 of cultured cells, 10-20 mg of bacteria. |
K0512 |
Genomic DNA Purification Kit
从全血中提取基因组DNA。
- Features
-
- 高效 一 0.2 ml血液中可提取2-10 μg基因组DNA。
- 快速 一 抽提过程仅需20分钟。
- 通用 一 从多种标本提取基因组DNA。
- 灵活 一 提取规模很容易放大和缩小。
- 安全 一 不含苯酚抽提操作。
- Applications
-
- 从以下材料抽提高质量基因组DNA:
一 全血或血清;
一 细胞培养液;
一 植物组织;
一 哺乳动物组织;
一 上皮细胞标本;
一 细菌。
- 从以下材料抽提高质量基因组DNA:
- Lysis Solution
- Precipitation Solution
- NaCl Solution
- 详细的操作手册
琼脂糖凝胶(0.7%)电泳分析未酶切的 (奇数泳道)和EcoRI (#ER0271)酶切的(偶数泳道) DNA样本。
M – Lambda DNA/HindIII Marker
1, 2 – 人血液标本
3, 4 – 人淋巴细胞
5, 6 – 人胎盘
7, 8 – 小鼠肝
9, 10 – Hedera helix
11, 12 – Haemophilus influenzae
13, 14 – E.coli ER2267
15, 16 – Bacillus subtilis
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文献和实验Reproducible purification of DNA and RNA in 96-well format. Genomic DNA and total RNA were purified from mouse tissues using the AllPrep DNA/RNA 96 Kit. A Purified DNA samples were analyzed on a 0.8% agarose gel. B
quandries on Genomic DNA purification-Molecular Biology
What is a crystaline precipitate that I get sometimes (not always) when I precipitate my genomic DNA preps and how can I get rid of it? theories are protein(that was not completely removed during extraction), salt(I use 3.5M KOAc pH 5.2) or SDS
Purification of Genomic DNA Using PureLink Silica Columns
different tubes. Before Starting Add 40 ml 96-100% ethanol to 10 ml Wash Buffer (W5) included with the kit. Store the Wash Buffer (W5) with ethanol at room temperature. Binding DNA 1. Remove a PureLink™ Spin Cartridge
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